Recombinant Mouse Carboxylesterase 1D (Ces1d)

Code CSB-YP844995MO
MSDS
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Source Yeast
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Code CSB-EP844995MO
MSDS
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Source E.coli
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Code CSB-EP844995MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP844995MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP844995MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ces1d
Uniprot No.
Alternative Names
Ces1d; Ces1; Ces3Carboxylesterase 1D; Carboxylesterase 3; EC 3.1.1.1; EC 3.1.1.67; Fatty acid ethyl ester synthase; FAEE synthase; Triacylglycerol hydrolase; TGH
Species
Mus musculus (Mouse)
Expression Region
19-565
Target Protein Sequence
YP SSPPVVNTVK GKVLGKYVNL EGFTQPVAVF LGVPFAKPPL GSLRFAPPQP AEPWSFVKNT TSYPPMCSQD AVGGQVLSEL FTNRKENIPL QFSEDCLYLN IYTPADLTKN SRLPVMVWIH GGGLVVGGAS TYDGLALSAH ENVVVVTIQY RLGIWGFFST GDEHSRGNWG HLDQVAALRW VQDNIANFGG NPGSVTIFGE SAGGFSVSVL VLSPLAKNLF HRAISESGVS LTAALITTDV KPIAGLVATL SGCKTTTSAV MVHCLRQKTE DELLETSLKL NLFKLDLLGN PKESYPFLPT VIDGVVLPKA PEEILAEKSF STVPYIVGIN KQEFGWIIPT LMGYPLAEGK LDQKTANSLL WKSYPTLKIS ENMIPVVAEK YLGGTDDLTK KKDLFQDLMA DVVFGVPSVI VSRSHRDAGA STYMYEFEYR PSFVSAMRPK AVIGDHGDEI FSVFGSPFLK DGASEEETNL SKMVMKFWAN FARNGNPNGG GLPHWPEYDQ KEGYLKIGAS TQAAQRLKDK EVSFWAELRA KESAQRPSHR EHVEL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Major lipase in white adipose tissue. Involved in the metabolism of xenobiotics and of natural substrates. Hydrolyzes triacylglycerols and monoacylglycerols, with a preference for monoacylglycerols. The susceptibility of the substrate increases with decreasing acyl chain length of the fatty acid moiety. Catalyzes the synthesis of fatty acid ethyl esters. Hydrolyzes retinyl esters.
Gene References into Functions
  1. the carboxylesterase 1d (Ces1d) and carboxylesterase 1e (Ces1e) expression and the capacity of hydrolytic activity of liver and intestine decreased, whereas the Akt/mTOR/HIF-1alpha/ Stra13 (DEC1) signaling was activated in type 2 diabetes mice. PMID: 25801056
  2. Inhibition of TGH activity ameliorates atherosclerosis development and improves insulin sensitivity in Ldlr(-/-) mice. PMID: 22872154
  3. TGH-mediated changes in cytosolic lipid droplet dynamics, were investigated. PMID: 20410140
  4. Loss of TGH/Ces3 in mice decreases blood lipids, improves glucose tolerance, and increases energy expenditure. PMID: 20197051
  5. Carboxylesterase 3 is an adipocyte lyase PMID: 15220344
  6. C/EBPalpha participates directly in the regulation of TGH, an enzyme associated with lipolysis PMID: 15752068

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Subcellular Location
Endoplasmic reticulum lumen. Cytoplasm, cytosol. Lipid droplet. Microsome.
Protein Families
Type-B carboxylesterase/lipase family
Tissue Specificity
Highest expression occurs in liver with lower levels in adipose tissue, kidney, heart, intestine, lung, testis and thymus.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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