Product Details

Purity

>85% (SDS-PAGE)

Target Names

Bhlhe41

Uniprot No.

Q99PV5

Alternative Names

Bhlhe41; Bhlhb3; Dec2; Class E basic helix-loop-helix protein 41; bHLHe41; Class B basic helix-loop-helix protein 3; bHLHb3; Differentially expressed in chondrocytes protein 2; mDEC2

Species

Mus musculus (Mouse)

Expression Region

1-410

Target Protein Sequence

MDEGIPHLQE RQLLEHRDFI GLDYSSLYMC KPKRSLKRDD TKDTYKLPHR LIEKKRRDRI NECIAQLKDL LPEHLKLTTL GHLEKAVVLE LTLKHLKALT ALTEQQHQKI IALQNGERSL KSPVQADLDA FHSGFQTCAK EVLQYLARFE SWTPREPRCA QLVSHLHAVA TQLLTPQVPS GRGSGRAPCS AGAAAASGPE RVARCVPVIQ RTQPGTEPEH DTDTDSGYGG EAEQGRAAVK QEPPGDSSPA PKRPKLEARG ALLGPEPALL GSLVALGGGA PFAQPAAAPF CLPFYLLSPS AAAYVQPWLD KSGLDKYLYP AAAAPFPLLY PGIPAAAAAA AAAAFPCLSS VLSPPPEKAG ATAGAPFLAH EVAPPGPLRP QHAHSRTHLP RAVNPESSQE DATQPAKDAP

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11.

Gene References into Functions

Bhlhe41 directly repressed the expression of cell-cycle regulators and inhibitors of BCR signaling while enabling pro-survival cytokine signaling. Thus, Bhlhe41 controls the development, BCR repertoire and self-renewal of B-1a cells. PMID: 28250425
Data show that the bHLH transcription factors SHARP1 and SHARP2 are involved in cognitive processing by controlling insulin-like growth factor II (Igf2) expression and associated signaling cascades. PMID: 26100875
abnormal sleep and certain (endo)phenotypes of psychiatric diseases may be caused by common mechanisms involving components of the molecular clock including SHARP1 and SHARP2. PMID: 25340473
SENP1 enhances adipogenesis through de-SUMOylation of Sharp-1, which then releases Sharp-1 repression of PPARgamma expression and adipocyte differentiation. These results reveal SENP1 as a novel regulator in adipogenesis. PMID: 24942744
DEC2 regulates cellular function by modulating the expression of Twist1 PMID: 25446074
These results demonstrate that Sharp-1 regulates muscle regenerative capacity, at least in part, by modulation of TGF-beta signaling PMID: 24357723
Data show that G9a, a lysine methyltransferase, is involved in Sharp-1-mediated inhibition of muscle differentiation. PMID: 23087213
Interactions of Per1/2 and Dec2 in the regulation of period, phase, and rhythm sustainment are cell-type specific. PMID: 23223373
RORalpha suppresses adipogenic differentiation at a later stage of differentiation by RORE-mediated stimulation of Dec1 and Dec2 expression. PMID: 22244086
Results suggest a partially redundant and bidirectional regulatory function for Dec1/2 genes in transcriptional translational feedback loops and conservation of Per1-Dec synergism between vertebrate and invertebrate clocks. PMID: 22215611
measured circadian locomotor behavior and clock gene expression in the SCN of Per2/Dec1/2 double- and triple-mutant mice to analyze the functional interaction of PER2 and DEC1/2 feedback on circadian pacemaker function in the SCN PMID: 22080784
Studies identify a novel function for Sharp-1 in cell cycle arrest and DNA damage-induced apoptosis. Inappropriate Sharp-1 expression may therefore be associated with tumorigenesis. PMID: 20006609
mSharp-1 may regulate the differentiation of several cell types during vertebrate development PMID: 12175508
Dec1 and Dec2 are regulators of the mammalian molecular clock, and form a fifth clock-gene family. PMID: 12397359
functions as a transcriptional repressor of E box activity and Stra13 expression PMID: 12657651
Dec1 and Dec2 are upregulated by clock protein PMID: 15038852
interference with MyoD function underlies Sharp-1-mediated repression of myogenic differentiation PMID: 15448136
Marked changes in the Dec1 and Dec2 expression suggest that CLOCK plays a major role in the expression of these genes in most tissues. PMID: 16267380
These findings demonstrate that (57)Arg in the basic region of DEC2 is essential for its activity in suppressing CLOCK/BMAL2-mediated transactivation. PMID: 16685415
Dec2 employs multiple mechanisms, including DNA-binding and protein-protein interactions, to achieve E-box-dependent transcriptional repressions. PMID: 17487425
DEC2, but not DEC1, suppressed VEGF gene expression under hypoxic conditions. PMID: 18233956
These observations collectively support the notion that overexpression of Stra13 and Dec2 links hypoxia signalling to altered circadian clock gene expression. PMID: 18342625
DEC1, along with DEC2, plays a role in the finer regulation and robustness of the molecular clock CLOCK/BMAL1 PMID: 18411297
Sharp-1 and Sharp-2 have roles as negative regulators of the molecular clock PMID: 18648504
SHARP1 inhibits adipogenesis through the regulation of C/EBP activity, which is essential for PPARgamma-ligand-dependent displacement of co-repressors from adipogenic promoters PMID: 19029947
the enhanced Th2 development is at least in part due to substantial up-regulation of CD25 expression elicited by Dec2, thereby resulting in hyperresponsiveness to IL-2 stimulation. PMID: 19880450
show that the basic helix-loop-helix transcription factor Dec2 was selectively expressed in TH2 cells. PMID: 19881507

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Subcellular Location

Nucleus.

Tissue Specificity

Expressed in skeletal muscle, brain and lung.

Database Links

KEGG: mmu:79362

STRING: 10090.ENSMUSP00000032386

UniGene: Mm.154529

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Recombinant Mouse Class E basic helix-loop-helix protein 41 (Bhlhe41)

Product Details

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Target Background

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