Recombinant Mouse Cytosolic phospholipase A2 beta (Pla2g4b)

Code CSB-YP018098MO
MSDS
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Source Yeast
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Code CSB-EP018098MO
MSDS
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Source E.coli
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Code CSB-EP018098MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018098MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP018098MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pla2g4b
Uniprot No.
Alternative Names
Pla2g4b; Cytosolic phospholipase A2 beta; cPLA2-beta; EC 3.1.1.4; Phospholipase A2 group IVB
Species
Mus musculus (Mouse)
Expression Region
1-782
Target Protein Sequence
MQAKVPETCL LTVRVLRASG LPSKDLVTSS DCYVTLNLPT ASSHTLQTRT VKNSRNPVWN QNFHFRIHRQ LKNVMELKVF DHDLVTRDDP VLSVLFDVGT LQIGTQRQSF SLGTQEKGCL EVEFRLQTLT DCEEQLISNG IVVARELSCL HVELKRTGDP KRSERKVQLV VAGACEGPQD ASAGTGSFHF HYPACWEQEL NVHLQDDPHE QLKVPLRTLP SSQLVRLVFP TSQEPLMRLE LKKEEGPKEL AVRLGCGPCP EEQAFLSKRK QVVAAALKKA LQLDQDLHED EIPVIAVMAT GGGIRAMTSL YGQLAGLQEL GLLDCISYIT GASGSTWALA NLYEDPEWSQ KDLAGPTEVL KTQVTKSKLG ALAPSQLWRY RQELAERARL GHPTCFTNLW ALINEALLHD KPHEHKLSDQ REALSRGQNP LPIYCALNSK EQGLSTFDFG EWCEFSPYEV GFPKYGAFIS SELFGSEFFM GRLVKQLPES RICFLEGIWS NLFAASLQDS LYWSSEPSQF WDRWAQDQAN LDKEQVPHLK IAEPPTMAGR IAELFTDLLT KRPLAHATHN FTRGLHFHKD YFQNSHFSAW KASKLDRLPN QLTPTEPHLC LLDVGYLINT SCPPLLQPTR DVDLILSLDY NLYGAFQQLQ LLSRFCQEQG IPFPSISPSP EEQRQPQECH LFCDPAQPEA PAVLHFPLVN DSFQDYSAPG VPRTSEEKAA GEVNLSSSDS PYHYTKVTYS QEDVDKLLRL THYNICNNQD RLREAMHQAV QRRRKRKQFR PE
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Calcium-dependent phospholipase A1 and A2 and lysophospholipase that may play a role in membrane phospholipid remodeling. Cleaves the ester bond of the fatty acyl group attached to the sn-1 or sn-2 position of phospholipids (phospholipase A1 and A2 activity, respectively), producing lysophospholipids that may be used in deacylation-reacylation cycles. The PLA1 versus PLA2 activity ratio appears to depend on the phospholipid headgroup, with mainly PLA2 activity toward anionic phospholipids such as phosphatidylglycerols. Hydrolyzes with high efficiency lysophospholipids enabling complete deacylation.
Gene References into Functions
  1. TLR2 cooperates with MyD88, PI3K, and Rac1 in lipoteichoic acid-induced cPLA2/COX-2-dependent airway inflammatory responses PMID: 20167866
Subcellular Location
Cytoplasm, cytosol. Mitochondrion membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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