Recombinant Mouse DNA ligase 4 (Lig4), partial

1. The data firmly demonstrate Lig4(R278H) activity renders nonhomologous end-joining (NHEJ) DNA repair to be more error-prone, and predict increased error-prone NHEJ and A-EJ suppression as the cause of defective B lymphopoiesis in Lig4 patients. PMID: 26608917
2. To promote homology-directed r at the expense of nonhomologous end joining , we targeted DNA ligase IV, a key enzyme in the NHEJ pathway, using the inhibitor Scr7. This approach should be applicable to other customizable endonucleases PMID: 25798939
3. Lig4 and XRCC1 double-deficient cells switch as efficiently as Lig4-deficient cells, clearly indicating that XRCC1 is dispensable for A-EJ in CH12F3 cells during class switch recombination PMID: 22392994
4. These studies provide insight into the interplay between DNA damage responses in the developing brain and the DNA ligase IV plat the role in repairing endogenously arising DNA double-strand breaks. PMID: 21734301
5. Lig4-deficient B cells have reduced, but still substantial, immunoglobulins class switch recombination PMID: 20142431
6. Homozygous DNA ligase IV R278H mutation in mice leads to leaky SCID and represents a model for human LIG4 syndrome. PMID: 20133615
7. Lig4 and Rad54 cooperate to support cellular proliferation, dna repair, and prevent chromosome and single chromatid aberrations PMID: 15175260
8. LIG4 mutations can result in either a developmental defect with immunological abnormalities or a severe combined immunodeficiency picture with normal development. PMID: 16357942
9. DNA Ligase IV is engaged in extrachromosomal circular major satellite DNA synthesis PMID: 16547499
10. Results describe the activity requirements for DNA ligases III and IV in the pathways of non-homologous DNA end joining. PMID: 17492771
11. Data show that disruption of the DNA ligase IV gene in a B cell line severely inhibits the initial rate of class switch recombination and causes a late cell proliferation defect under cytokine stimulation. PMID: 19001141
12. particular features of the c-Myc locus select it as a preferential translocation/amplification target, compared to the endogenous N-myc locus, in Lig4/p53-deficient pro-B cell lymphomas PMID: 19174520
13. the Lig4Y288C mutation leads to multiple defects in lymphocyte development and function, including impaired V(D)J recombination, peripheral lymphocyte survival and proliferation, and B cell class switch recombination PMID: 19451691

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KEGG: mmu:319583

STRING: 10090.ENSMUSP00000093130

UniGene: Mm.80584