Recombinant Mouse Delta (24)-sterol reductase (Dhcr24), partial

Code CSB-YP844991MO
MSDS
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Source Yeast
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Code CSB-EP844991MO
MSDS
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Source E.coli
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Code CSB-EP844991MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP844991MO
MSDS
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Source Baculovirus
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Code CSB-MP844991MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dhcr24
Uniprot No.
Alternative Names
Dhcr24; Kiaa0018; Delta(24)-sterol reductase; EC 1.3.1.72; 24-dehydrocholesterol reductase; 3-beta-hydroxysterol Delta-24-reductase
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. In addition to its cholesterol-synthesizing activity, can protect cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis.
Gene References into Functions
  1. When DHCR24 is genetically deleted there is a decreased association of EAAT2 with lipid rafts after ischemia. PMID: 26628388
  2. Data (including data from transgenic mice) suggest that expression of Dhcr24 is down-regulated in brain tissues of mice exhibiting symptoms modeling advanced Huntington's disease. PMID: 24916565
  3. RE1-silencing transcription factor acts because it is bound in close proximity to SREBP, thus amplifying its ability to upregulate 24-dehydrocholesterol reductase PMID: 24861183
  4. concluded that TH up-regulates Seladin-1 gene expression at the transcriptional level and LXR-alpha maintains the gene expression PMID: 23359226
  5. This is the first demonstration that DHCR24 plays an important role in long bone growth by protecting chondrocytes from reactive oxygen species. PMID: 21845517
  6. Seladin-1 is shown to be an LPS-responsive gene that negatively regulates the TNF- alpha production and bone resorption in response to LPS. PMID: 20406300
  7. Seladin-1-dependent cholesterol synthesis is involved in lowering Abeta levels. PMID: 16407971
  8. cellular cholesterol biosynthesis is critical for the activation and maintenance of the Akt-Bad cell survival cascade in response to growth factors such as insulin. PMID: 16513830
  9. Cholesterol homeostasis in Dhcr24 knockout mice is characterized by strong activation of liver X receptor-targeted genes and by mechanisms compensating for cholesterol depletion. PMID: 17761942
  10. ablation of DHCR24/seladin-1 prevented apoptosis of primary neurons in a p53-dependent manner PMID: 17984220
  11. DHCR24 plays important roles for normal development of epidermis and hair follicle even in postnatal life. PMID: 19542918

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Subcellular Location
Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein.
Protein Families
FAD-binding oxidoreductase/transferase type 4 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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