Recombinant Mouse Deoxyhypusine hydroxylase (Dohh)

Code CSB-YP859555MO
MSDS
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Source Yeast
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Code CSB-EP859555MO
MSDS
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Source E.coli
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Code CSB-EP859555MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP859555MO
MSDS
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Source Baculovirus
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Code CSB-MP859555MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dohh
Uniprot No.
Alternative Names
DohhDeoxyhypusine hydroxylase; DOHH; EC 1.14.99.29; Deoxyhypusine dioxygenase; Deoxyhypusine monooxygenase
Species
Mus musculus (Mouse)
Expression Region
1-302
Target Protein Sequence
MVTEQEIEAI GKTLVDPKQP LQARFRALFT LRGLGGPDAI SWISRGFEDS SALLKHELAY CLGQMRDARA IPVLADVLQD TSQEPMVRHE AGEALGAIGN PEVLGLLKQY STDPVVEVAE TCQLAVGRLE WLQQHPGEAT CAGPYLSVDP APPAAEQDVG RLREALLDEA RPLFERYRAM FALRNVGGKE AALALAEGLQ CGSALFRHEV GYVLGQLQHE AAVPGLAATL ARTTESPMVR HECAEALGAI ARPACLAALR EHIEDPEQVV RESCEVALDM YEYESSQDFQ YADGLERLRP PP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.
Gene References into Functions
  1. Deletion of hypusine modification enzymes ( deoxyhypusine synthase and deoxyhypusine hydroxylase )exerts strong phenotypes. eIF-5A2-deleted animals are viable and fertile. PMID: 26037925
  2. An evolutionarily conserved role for the DOHH-mediated second step of hypusine synthesis in early embryonic development, is reported. PMID: 24832488
Protein Families
Deoxyhypusine hydroxylase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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