Name: Recombinant Mouse Deoxyribonuclease-2-beta (Dnase2b)
Brand: CUSABIO
SKU: 879452MO

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Dnase2b

Uniprot No.

Q9QY48

Alternative Names

Dnase2b; DladDeoxyribonuclease-2-beta; EC 3.1.22.1; DNase II-like acid DNase; DNase2-like acid DNase; Deoxyribonuclease II beta; DNase II beta; Endonuclease DLAD

Species

Mus musculus (Mouse)

Expression Region

23-354

Target Protein Sequence

TPEISCRN EYGEAVDWFI FYKLPKRTSK ASEEAGLQYL YLDSTRQTWN KSLYLINSTR SALGRTLQHL YDTHNSTNDT AYLIYNDGVP GSVNYSRQYG HAKGLLVWNR TQGFWLIHSV PKFPPVHGYE YPTSGRRYGQ TGICITFGYS QFEEIDFQLL VLQPNIYSCF IPSTFHWKLI YMPRMCANSS SLKIPVRYLA ELHSAQGLNF VHFAKSSFYT DDIFTGWIAQ KLKTHLLAQT WQKKKQELPS NCSLPYHVYN IKSIGVTSKS YFSSRQDHSK WCVSIKGSAN RWTCIGDLNR SLHQALRGGG FICTKNHYIY QAFHKLYLRY GFCK

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

Hi! I'm a post-doc at the University of Parma, Italy. I managed to purify recombinant human DNASE2B from E. coli, but without any success. For the purification strategy, I chose His tag and Cobalt affinity chromatrography. May I ask you how you manage to produce this protein? Every information you could give me will be very helpful for me! I thank you in advance.

Regarding the purification issue, did you only perform His tag and Cobalt affinity chromatrography purificaiton ?
Have you tried to optimize in multiple conditions, for example, to further optimize the vector, culture conditions or try some other secondary purification methods, etc.
Our article for your reference: https://www.cusabio.com/c-20275.html
Choosing an appropriate purification method is required:
For some applications, a crude extract is sufficient. However, for other uses, a high level of purity is required. To produce the protein with high purity, purifying the protein produced is necessary. Protein purification refers to a process that isolates one or more proteins from cells, tissues or whole organisms. A variety of purification strategies has been developed, such as size exclusion chromatography (SEC), separation based on charge or hydrophobicity, affinity chromatography (AC), and HPLC.
Sorry, as we haven't expressed this protein before, we are not sure if there is some special problem during the purification, some proteins are easy to purify, but others are not, which need further optimization according to our experience. Sorry, for the time being, we can't share any useful experience specific to this protein.
Probably you can try to optimize in multiple condition as we mentioend above.

Target Background

Function

Hydrolyzes DNA under acidic conditions. Does not require divalent cations for activity. Participates in the degradation of nuclear DNA during lens cell differentiation.

Gene References into Functions

These data identify DNase 2 as the predominant DNase on the mammalian skin surface and indicate that its activity is primarily targeted to exogenous DNA. PMID: 21390259
gene lie head-to-head with the urate oxidase gene PMID: 11700027
The DLAD-/- mice develop cataracts of the nucleus lentis, and their response to light on electroretinograms is severely reduced; these results indicate that DLAD is responsible for the degradation of nuclear DNA during lens cell differentiation PMID: 12944971
DNase II-like acid DNase is synthesized as a precursor with a signal sequence, and is localized to lysosomes. DNase II-like acid DNase mRNA was found in cortical fiber cells but not epithelial cells PMID: 17509075

Subcellular Location

Lysosome.

Protein Families

DNase II family

Tissue Specificity

Highly expressed in the eye lens. Detected in liver, but not in the other tissues tested.

Database Links

KEGG: mmu:56629

STRING: 10090.ENSMUSP00000029836

UniGene: Mm.25853

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Recombinant Mouse Deoxyribonuclease-2-beta (Dnase2b)

Product Details

Customer Reviews and Q&A

Target Background

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