Recombinant Mouse Dynein intermediate chain 1, axonemal (Dnai1)

Code CSB-YP806948MO
MSDS
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Source Yeast
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Code CSB-EP806948MO
MSDS
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Source E.coli
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Code CSB-EP806948MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP806948MO
MSDS
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Source Baculovirus
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Code CSB-MP806948MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dnai1
Uniprot No.
Alternative Names
Dnai1; Dnaic1Dynein intermediate chain 1; axonemal; Axonemal dynein intermediate chain 1
Species
Mus musculus (Mouse)
Expression Region
1-701
Target Protein Sequence
MPSKQIRKQS ISVTRGARRR DEDSGTDVGE GTDEWSQSKA TVRPPDQLEL TDAELKEEFT RILTANNPHA PQNIVRYSFK EGTYKLIGFV NQMAVHFSQV GNLIPKDSDE GRRQHYRDEM VAGSQESIKV VTSEAENLEE EEEPKEGEGE AEAEAEAGSQ TDIPAAAETT EKVIEEELMA PVQPKERKLT NQFNFSERAS QTFNNPLRDR ECQMEPPPRT NFSATANQWE IYDAYVDELE KQEKTKEKEK AKTPVAKKTE KMAMRKLTSM ESQSDDITKV TQAAKIVERM VNQNTYDDVA QDFKYYEDTA DEYRDQEGTL LPLWKFQNDK AKRLAVTALC WNPKYKDLFA VGHGSYDFMK QSRGMLLLYS MKNPSFPEYM FSSESGIMCL DVHVDHPYLV VVGYYDGNVA IYNLKKPHSQ PCFRSTSKSG KHTDPVWQVK WQKDDMDHNL NFFSVSSDGR IVSWTLVKSE LVHIDIIKLK TEGSTTEIPE GLQLHTVGCG TAFDFHKEID YMFLVGTEEG KIYKCSKSYS SQFLDTYDAH NMAVDAVLWN PYHTKVFMSC SSDWTVKIWD HTIKTPMFIY DLNSAVGDVA WAPYSSTVFA AVTTDGKAHV FDLAVNKYEA ICNQPVVAKK KNKITHVQFN PIHPIIIVGD DRGHIICLKL SPNLRKMPKE KKGQEVQKGP AVEIAKLDKL LNLVREVKTK T
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Part of the dynein complex of respiratory cilia.
Gene References into Functions
  1. Dnah6 genetically interacts with Dnai1 and Dnah5 to cause heterotaxy and primary ciliary dyskinesia. PMID: 26918822
  2. Motile nodal cilia patterns heart looping but heart and visceral organ lateralization is driven by signaling not requiring nodal cilia motility. Dnaic1 mutants can achieve situs solitus or inversus even with immotile nodal cilia. PMID: 22408017
  3. dnaic1 deletion in a murine model of primary ciliary dyskinesia causes chronic rhinosinusitis PMID: 19675306
Subcellular Location
Cytoplasm, cytoskeleton, cilium axoneme.
Protein Families
Dynein intermediate chain family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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