Recombinant Mouse E3 ubiquitin-protein ligase RNF138 (Rnf138)

Code CSB-YP887422MO
MSDS
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Source Yeast
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Code CSB-EP887422MO
MSDS
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Source E.coli
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Code CSB-EP887422MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887422MO
MSDS
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Source Baculovirus
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Code CSB-MP887422MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rnf138
Uniprot No.
Alternative Names
Rnf138; Trif; E3 ubiquitin-protein ligase RNF138; EC 2.3.2.27; RING finger protein 138; RING-type E3 ubiquitin transferase RNF138
Species
Mus musculus (Mouse)
Expression Region
2-245
Target Protein Sequence
SEELSAATS YTEDDFYCPV CQEVLKTPVR TAACQHVFCR KCFLTAMRES GIHCPLCRGS VTRRERACPE RALDLENIMR RFSGSCRCCS KKIKFYRMRH HYKSCKKYQD EYGVSSVIPN FKISQDSVRS SNRSETSASD NTETYQEDTS SSGHPTFKCP LCQESNFTRQ RLLDHCNSNH LFQIVPVTCP ICVSLPWGDP SQITRNFVSH LNQRHQFDYG EFVNLQLDEE TQYQTAVEES FQVNM
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
E3 ubiquitin-protein ligase involved in DNA damage response by promoting DNA resection and homologous recombination. Recruited to sites of double-strand breaks following DNA damage and specifically promotes double-strand break repair via homologous recombination. Two different, non-exclusive, mechanisms have been proposed. According to a report, regulates the choice of double-strand break repair by favoring homologous recombination over non-homologous end joining (NHEJ): acts by mediating ubiquitination of XRCC5/Ku80, leading to remove the Ku complex from DNA breaks, thereby promoting homologous recombination. According to another report, cooperates with UBE2Ds E2 ubiquitin ligases (UBE2D1, UBE2D2, UBE2D3 or UBE2D4) to promote homologous recombination by mediating ubiquitination of RBBP8/CtIP. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.
Gene References into Functions
  1. Rnf138 deficiency promotes the apoptosis of spermatogenic cells. PMID: 28518149
Subcellular Location
Chromosome.
Database Links
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