Recombinant Mouse E3 ubiquitin-protein ligase TRIM31 (Trim31)

Code CSB-YP854489MO
MSDS
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Source Yeast
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Code CSB-EP854489MO
MSDS
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Source E.coli
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Code CSB-EP854489MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP854489MO
MSDS
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Source Baculovirus
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Code CSB-MP854489MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Trim31
Uniprot No.
Alternative Names
Trim31; E3 ubiquitin-protein ligase TRIM31; EC 2.3.2.27; RING-type E3 ubiquitin transferase TRIM31; Tripartite motif-containing protein 31
Species
Mus musculus (Mouse)
Expression Region
1-507
Target Protein Sequence
MAGQPLACQL QEEVTCPICM EILQDPVTID CGHNFCLQCI SQVGKTSEKI QCPLCKLSVN KNTFRPNKLL ASLAEKIQSM DPADIQAEKE DSRCQRHKEK LHYFCEQDGA FLCVVCRDSK DHKSHNVTLI DEAAQNYKVQ IESQAQDLGQ KDKKIIEEKK QGEGAIWAFR AQVDLEKLKI HEEFKLLRQR LDEEESFLLS RLDWLEQQGA KQLRQYVTVT EKQLNSLRKL TKSLKIRLQS SSMELLKDIK DALSRGKEFQ FLNPNPVPED LEKKCSEAKA RHESIIKTLT ELKDDMNAEG KRDKSAFMNS LNKEEKESWS LLQKNNSVLP TSVPVTLDKS SADPDLTFSQ DLKKVTLYIV AGKASNRQAK PRPFYPFHCV RGSPGLSSGR QVWEAEIRGP SGGACIVGVV TELARGAQSQ TVSAQSYIWA LRISPSGCQP FTNCKAQEYL QVCLKKVGVY VNHDCGEVVF YDAITSKHIY TFQTSFDGKV FPLFGLQVAC SHITLSP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May have E3 ubiquitin-protein ligase activity. Regulator of Src-induced anchorage independent cell growth.
Gene References into Functions
  1. this paper identifies TRIM31, an E3 ubiquitin ligase of the TRIM family of proteins, as a regulator of MAVS aggregation PMID: 27992402
  2. TRIM31 attenuates c-Src signaling via p52(Shc) under anchorage-independent growth conditions and is potentially associated with growth activity of cells in the gastrointestinal tract. PMID: 19665990
Subcellular Location
Cytoplasm. Mitochondrion.
Tissue Specificity
Highly expressed in the gastrointestrinal tract, with high expression in the small intestine, moderate in the large intestine and weak in the stomach and esophagus.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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