Recombinant Mouse FYVE, RhoGEF and PH domain-containing protein 2 (Fgd2)

Code CSB-YP806932MO
MSDS
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Source Yeast
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Code CSB-EP806932MO
MSDS
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Source E.coli
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Code CSB-EP806932MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP806932MO
MSDS
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Source Baculovirus
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Code CSB-MP806932MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Fgd2
Uniprot No.
Alternative Names
Fgd2; FYVE; RhoGEF and PH domain-containing protein 2
Species
Mus musculus (Mouse)
Expression Region
1-655
Target Protein Sequence
MERACEKQDS VCNLVAVFEN NRTPGEAPGS HSLEDQPHSP EHQLSLSPEP WEAPPVKEAL KSEFRPVSRT YLSSLKNKLS SGAWRRSCQP GVSPGPETQE PEEKRVVREL LETEQAYVAR LHLLDQVFFQ ELLREAGRSK AFPEDVVKLI FSNISSIYRF HAQFFLPELQ RRVDDWAATP RIGDVIQKLA PFLKMYSEYV KNFERAAELL ATWMDKSQPF QEVVTRIQCS EASSSLTLQH HMLEPVQRIP RYELLLKEYV QKLPAQAPDL EDAQRALDMI FSAAQHSNAA IAEMERLQGL WDVYQRLGLE DDIVDPSNTL LREGPVLKIS FRRSDPMERY LVLFNNMLLY CVPRVLQVGA QFQVRTRIDV AGMKVRELTD AEFPHSFLVS GKQRTLELQA RSRDEMVSWM QACQAAIDQV EKRSETFKAA VQGPQGDTQE PKPQVEELGL RAPQWVRDKM VTMCMRCQEP FNALTRRRHH CRACGYVVCA KCSDYRAELK YDSNRPNRVC LTCYTFLTGN VLPQGKEDKR RGILEKEASA APEQSLVCSF LQLIGDKCSR SLPRSWCVIP RDDPLVLYVY AAPQDTKAHT SIPLLGYQVI SGPQGDPRVF QLQQSGQQYT FKAESVELQG RWVTAIKRAA SGRTPEGPDE EDVSD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Activates JNK1 via CDC42 but not RAC1. Binds to phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 5-monophosphate, phosphatidylinositol 4-monophosphate and phosphatidylinositol 3-monophosphate.
Gene References into Functions
  1. The t allele of Fgd2 is overexpressed in testis compared with wild type, a loss-of-function allele of Fgd2 generated by gene targeting reduces the transmission ratio of the t haplotype t(h49), directly demonstrating the role of Fgd2 as Distorter. PMID: 17234881
Subcellular Location
Cytoplasm. Nucleus. Early endosome. Early endosome membrane. Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Note=Recruitment to the endosome and ruffle membrane requires the presence of phosphoinositides.
Tissue Specificity
Lymph node, spleen, B-lymphocytes and macrophages (at protein level). Expressed at high levels in lymph node, spleen, B-lymphocytes and bone marrow macrophages. Expressed at lower levels in mature bone marrow dendritic cells. In both immature and mature B
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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