Recombinant Mouse Factor in the germline alpha (Figla)

Code CSB-YP008675MO
MSDS
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Source Yeast
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Code CSB-EP008675MO
MSDS
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Source E.coli
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Code CSB-EP008675MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP008675MO
MSDS
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Source Baculovirus
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Code CSB-MP008675MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Figla
Uniprot No.
Alternative Names
FiglaFactor in the germline alpha; FIGalpha; Transcription factor FIGa
Species
Mus musculus (Mouse)
Expression Region
1-194
Target Protein Sequence
MDTAPASPEP FLVTPQAEVL EELIQAQMGP LPRLAAICRL KRLPSGGYST TDDLHLVLER RRVANAKERE RIKNLNRGFA KLKALVPFLP QSRKPSKVDI LKGATEYIQI LGCVLEEAKV SEKQSPEEQT HSGRPSDPHV SSTRELLGNA TQPTSCASGL KKEEEGPWAY AGHSEPLYSY HQSTVPETRS YFTH
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Germ-line specific transcription factor implicated in postnatal oocyte-specific gene expression. Plays a key regulatory role in the expression of multiple oocyte-specific genes, including those that initiate folliculogenesis and those that encode the zona pellucida (ZP1, ZP2 and ZP3) required for fertilization and early embryonic survival. Essential for oocytes to survive and form primordial follicles. The persistence of FIGLA in adult females suggests that it may regulate additional pathways that are essential for normal ovarian development. Binds to the E-box (5'-CANNTG-3') of the ZPs (ZP1, ZP2, ZP3) promoters.
Gene References into Functions
  1. GFP positive cells showed the expression of germ cell markers Vasa, meiotic specific gene Stra8, Scp3, oocyte markers Gdf9, Zp3 and Figla, indicating that this method could be used for the purification and selection of female germ cells PMID: 22213070
  2. Data suggest that a dynamic DNA CpG methylation in Sohlh2 and Figlalpha promoters, but not in the intron and exon sequences, is linked to regulation of gene expression, even though CpG islands are also present in their introns or exons. PMID: 21427160
  3. physiological expression of Figla plays a critical dual role in activation of oocyte-associated genes and repression of sperm-associated genes during normal postnatal oogenesis PMID: 20479125
  4. FIGLA is a central regulator of oocyte-specific genes that play roles in folliculogenesis, fertilization and early development. PMID: 17567914
Subcellular Location
Nucleus.
Tissue Specificity
Expressed only in the oocytes within the ovary and at lower level in the testis. Found in the resting oocytes of the primordial follicle cells, at the periphery of the ovary and in the hilar region. Also detected in growing oocytes, but at lower levels.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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