Recombinant Mouse Fanconi anemia group A protein homolog (Fanca), partial

Code CSB-YP862406MO
MSDS
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Source Yeast
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Code CSB-EP862406MO
MSDS
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Source E.coli
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Code CSB-EP862406MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP862406MO
MSDS
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Source Baculovirus
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Code CSB-MP862406MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Fanca
Uniprot No.
Alternative Names
FancaFanconi anemia group A protein homolog; Protein FACA
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
Gene References into Functions
  1. Fanca(-/-) mice showed a skewed Vkappa gene usage. PMID: 27883081
  2. FANCA safeguards interphase and mitosis during hematopoiesis PMID: 26366677
  3. study indicates that Fanca expression during endomitosis is crucial for normal megakaryopoiesis and platelet production. PMID: 25261197
  4. Data show that Fanconi anemia complementation group A Fanca is required for the induction of transition mutations at A/T residues during somatic hypermutation (SHM) and immunoglobulin (Ig) class switch recombination (CSR). PMID: 24799500
  5. CD25(+)Foxp3(+) Tregs of Fanca(-/-) or Fancd2(-/-) mice were less efficient in suppressing the production of GVHD-associated inflammatory cytokines. PMID: 24501220
  6. null mutations in Fanca or Fancg are fully epistatic PMID: 22036606
  7. genetic diversity in FANCA, FANCC and FANCL does not support an association of these genes with cervical cancer susceptibility in the Swedish population. PMID: 21543111
  8. The results support a model where both FANCA and FANCC are part of a multi-protein nuclear FA complex with identical function in cellular responses to DNA damage and germ cell survival. PMID: 12135664
  9. To study the in vivo role of the FA group A gene (Fanca), gene-targeting techniques were used to generate Fanca(tm1Hsc) mice in which Fanca exons 1-6 were replaced by a beta-galactosidase reporter construct. PMID: 12913077
  10. GnRH induced a rapid, transient increase in Fanca mRNA. PMID: 15128600
  11. Fanca protein as an integral component in the early step of homologous repair of DNA double-strand brearks thereby minimizing the genomic instability. PMID: 15905196
  12. Fanca knockout mice presented with microcephalies and a decreased neuronal production in developing cortex and adult brain, demonstrating a critical role for Fanconi pathway in neural stem and progenitor cells during developmental and adult neurogenesis. PMID: 18239686

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Subcellular Location
Nucleus. Cytoplasm. Note=The major form is nuclear. The minor form is cytoplasmic.
Tissue Specificity
Mainly expressed in testis and lymphoid tissues like thymus, lymph nodes, and spleen, and at lower levels in kidney and ovary.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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