Recombinant Mouse Formin-2 (Fmn2), partial

Code CSB-YP864361MO
MSDS
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Source Yeast
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Code CSB-EP864361MO
MSDS
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Source E.coli
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Code CSB-EP864361MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP864361MO
MSDS
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Source Baculovirus
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Code CSB-MP864361MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Fmn2
Uniprot No.
Alternative Names
Fmn2Formin-2
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Actin-binding protein that is involved in actin cytoskeleton assembly and reorganization. Acts as an actin nucleation factor and promotes assembly of actin filaments together with SPIRE1 and SPIRE2. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning, asymmetric oocyte division and polar body extrusion during female germ cell meiosis. Plays a role in responses to DNA damage, cellular stress and hypoxia by protecting CDKN1A against degradation, and thereby plays a role in stress-induced cell cycle arrest. Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage. Protects cells against apoptosis by protecting CDKN1A against degradation.
Gene References into Functions
  1. FlnA and Fmn2 are likely essential to cell proliferation, differentiation and cell death in a variety of tissues and organs, further reiterating the importance of vesicle trafficking in regulation of development PMID: 29240780
  2. Fmn2 mutant mice develop accelerated age-associated memory decline that is further increased in the presence of additional risk factors and is mechanistically linked to a loss of transcriptional homeostasis. PMID: 28768717
  3. Fmn2 as a mediator of actin bundle integrity, enabling efficient force transmission to the adhesion sites. PMID: 26718007
  4. Data suggest formin homology domain (FH2) of Fmn2 binds actin at filament barbed end as weak capper and plays a role in displacing WASP homology domain 2 (WH2) domains of Spire-1 from actin; competitive binding of Fmn2 vs Spire-1 aids actin assembly. PMID: 26668326
  5. Spire recruits Fmn2 and facilitates its association with actin filaments barbed ends. PMID: 24586110
  6. Results suggest that the regulation of microtubule acetylation is likely a general formin activity. PMID: 23110170
  7. analysis of the molecular basis of the Spir1/formin-2 interaction PMID: 21705804
  8. Spire1 and Spire2 cooperate with Formin-2 (Fmn2) to nucleate actin filaments in mouse oocytes. PMID: 21620703
  9. High formin 2 levels at meiosis I entry induce meshwork maintenance, leading to equal forces being exerted on the chromosomes, preventing spindle migration. PMID: 21653611
  10. Gene profiling of Fmn2 in Graffi murine leukemia virus-induced lymphoid leukemias suggest its important role in leukemogenesis. PMID: 21135260
  11. Fmn2 is a candidate in a QTL hotspot associated with neurobehavioral traits and gene expression in the mouse brain. Hippocampal pyramidal neurons exhibit distinct and fine granular immunoreactivity for FMN2. PMID: 19008955
  12. formin-2 is a maternal-effect gene that is expressed in oocytes and is required for progression through metaphase of meiosis I PMID: 12447394
  13. The mouse formin-2 gene has been found to be expressed in the developing nervous system and in neuronal cells of the adult brain. PMID: 15053972
  14. It is likely that human FMN2 has the same function as Fmn2 in the mouse (i.e., maintenance of the meiotic spindle). PMID: 15866570

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Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm, cytosol. Cytoplasm, perinuclear region. Nucleus. Nucleus, nucleolus. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Formin homology family, Cappuccino subfamily
Tissue Specificity
Detected in brain and in oocytes (at protein level). Expressed almost exclusively in the developing and mature central nervous system. Detected in oocytes.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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