Product Details

Purity

>85% (SDS-PAGE)

Target Names

Fv1

Uniprot No.

P70213

Alternative Names

Fv1Friend virus susceptibility protein 1

Species

Mus musculus (Mouse)

Expression Region

1-459

Target Protein Sequence

MNFPRALAGF SSWLFKPELA EDSPDNDSPD NDTVNPWREL LQKINVADLP DSSFSSGKEL NDSVYHTFEH FCKIRDYDAV GELLLAFLDK VTKERDQFRD EISQLRMHIN DLKASKCVLG ETLLSYRHRI EVGEKQTEAL IVRLADVQSQ VMCQPARKVS ADKVRALIGK EWDPVTWDGD VWEDIDSEGS EEAELPTVLA SPSLSEESGY ALSKERTQQD KADAPQIQSS TSLVTSEPVT RPKSLSDLTS QKHRHTNHEL NSLAHSNRQK AKEHARKWIL RVWDNGGRLT ILDQIEFLSL GPLSLDSEFN VIARTVEDNG VKSLFDWLAE AWVQRWPTTR ELQSPDTLEW YSIEDGIERL RELGMIEWLC VKATCPQWRG PEDVPITRAM RITFVRETRE TWKSFVFSLL CIKDITVGSV AAQLHDLIEL SLKPTAAGLT SVGSVGVLSL SPWKHQSNS

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Retroviral restriction factor that prevents infection by gammaretroviruses. Acts by interacting with the capsid protein ca after entry of the virus into the cell. This interaction presumably disrupt the capsid thereby inactivating the viral genome, making it unable to enter host nucleus and integrate into host genome.

Gene References into Functions

Regions of Fv1 adapt cooperatively, highlighting its preference for repeated structures and suggesting that this functionally constrained aspect of the retroviral capsid lattice presents a common target in the evolution of intrinsic immunity. PMID: 30224488
data indicate that for some but not all Fv1 proteins, gain of restriction activities could be achieved by increasing the expression level of Fv1; however such a concentration dependent effect is not seen with most Fv1s and cannot explain the recently reported activities against non-MLVs PMID: 27342974
These results support the hypothesis that Fv1 defended mice against waves of retroviral infection PMID: 24603659
The antiparallel organization of the NtD regions of Fv1 and Trim5alpha dimers correctly positions C-terminal specificity and N-terminal effector domains and facilitates stable binding to adjacent capsid hexamers in viral cores. PMID: 24979782
Ordered assembly of murine leukemia virus capsid protein on lipid nanotubes directs specific binding by the restriction factor, Fv1. PMID: 21436027
an examination of expression and intracellular localization of this protein PMID: 12667816
A variety of amino acid changes affecting Fv1 tropism were identified, at mouse leukemia virus CA protein positions 82, 92 to 95, 105, 114, and 117, and they all were mapped to the apparent exterior of virion-associated CA. PMID: 15331691
defined the time point at which Fv1 interacts with virus and shown that it can work additively and independently of TRIM5; conclude that Fv1 and TRIM5 can both recognize retroviral particles early after cell entry and before reverse transcription PMID: 16474118
A model for the arrangement of domains in Fv1 suggesting that dimerization of the N-terminal domain is necessary for Fv1 function to allow the protein to interact with multiple capsid protomers in retroviral cores was proposed. PMID: 16873278
Virus with deletion of 114His in the capsid gene failed to replicate in Fv1-expressing cells. PMID: 18467880

Hide All

Database Links

KEGG: mmu:14349

STRING: 10090.ENSMUSP00000092054

UniGene: Mm.422607

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-YP304889MO
MSDS	MSDS
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP304889MO
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP304889MO-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP304889MO
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP304889MO
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Mouse Friend virus susceptibility protein 1 (Fv1)

Product Details

Customer Reviews and Q&A

Target Background

×CloseMessage