Recombinant Mouse G2/M phase-specific E3 ubiquitin-protein ligase (G2e3)

Code CSB-YP713051MO
MSDS
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Source Yeast
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Code CSB-EP713051MO
MSDS
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Source E.coli
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Code CSB-EP713051MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP713051MO
MSDS
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Source Baculovirus
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Code CSB-MP713051MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
G2e3
Uniprot No.
Alternative Names
G2e3; Kiaa1333G2/M phase-specific E3 ubiquitin-protein ligase; EC 2.3.2.26; G2/M phase-specific HECT-type E3 ubiquitin transferase
Species
Mus musculus (Mouse)
Expression Region
1-716
Target Protein Sequence
MNENKPDNSQ SLACVFCRKN DDCPNKYGEK KTYEKWNFSV HYYCLLMSSG IWQRGKEEEG VYGFLIEDIR KEVQRASKLK CTVCKKNGAS IGCVVPTCKR SYHLPCGLQK ECIFQFTDNF ASFCWKHRPV QAITSNKYSS SLPCTICLEF VEPIPTYNIL QSPCCKNAWF HRDCLQVQAI NAGVFFFRCT LCNNTDIFQK EMLRMGIHIP EKDASWELEE NAYQELLQSH DRCDIRRCHC KKGRDYNEPN SKWEVKRCQS CGSSGTHLAC SSLQSWEQNW ECLDCRRITY TSDFQKAPKH PLANSTNVTV TDCLLEESSS KLPRQSTVAQ HKELLRQGSK FRRDISTILI ELGFQIKKKT KTLYINKANV WRSALEQFQS QKFNPSCSID VVYVNGNEVG SQHLGSKQEF LSHLMHHLEN SSVFEGSLAK NLSLNSQAVK ENLYYEVGKM LAISLVHGGP SPGFFSETLF NCLAYGPENT LPTLDDVSDI DVAQIIIKID SATDLNILNS VISQHYNYLE VSGCLRLTTS LSDKFMLVKD ILFYHVINRV KAPFESFKQG LKTLGVLEKI QTYPEAFYKI LCHKPENLSA KNLSDLFTIH SVADVQTLRF WNSYLKAIED GKSATTMEDI LIFATGCSSV PPTGFKPSLS VECLHVDFPV ADKYRNHLVL PATNTYEEFQ ENMDFTIRDT LRLEKEERSH ILPRTLNVSS NEEMLI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Required for prevention of apoptotic death in early embryogenesis.
Gene References into Functions
  1. G2E3 is a dual function ubiquitin ligase essential for prevention of apoptosis in early embryogenesis PMID: 18511420
Subcellular Location
Nucleus, nucleolus. Cytoplasm.
Tissue Specificity
In the developing embryo, expressed predominantly in the central nervous system and early limb bud. In the adult, highest expression in Purkinje cell bodies and cells lining the ductus deferens.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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