Recombinant Mouse Galectin-8 (Lgals8)

Code CSB-YP862404MO
MSDS
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Source Yeast
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Code CSB-EP862404MO
MSDS
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Source E.coli
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Code CSB-EP862404MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP862404MO
MSDS
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Source Baculovirus
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Code CSB-MP862404MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Lgals8
Uniprot No.
Alternative Names
Lgals8; Galectin-8; Gal-8; LGALS-8
Species
Mus musculus (Mouse)
Expression Region
1-316
Target Protein Sequence
MLSLNNLQNI IYNPIIPYVG TITEQLKPGS LIVIRGHVPK DSERFQVDFQ LGNSLKPRAD VAFHFNPRFK RSSCIVCNTL TQEKWGWEEI TYDMPFRKEK SFEIVFMVLK NKFQVAVNGR HVLLYAHRIS PEQIDTVGIY GKVNIHSIGF RFSSDLQSME TSALGLTQIN RENIQKPGKL QLSLPFEARL NASMGPGRTV VIKGEVNTNA RSFNVDLVAG KTRDIALHLN PRLNVKAFVR NSFLQDAWGE EERNITCFPF SSGMYFEMII YCDVREFKVA INGVHSLEYK HRFKDLSSID TLSVDGDIRL LDVRSW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy. Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture. Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52. Required to restrict infection of bacterial invasion such as S.typhimurium. Also required to restrict infection of Picornaviridae viruses. Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.
Gene References into Functions
  1. Galectin-8 regulates antibacterial autophagy through differential recognition of host glycans on damaged phagosomes. PMID: 29800364
  2. Galectin-8 activates dendritic cells and stimulates antigen-specific immune response elicitation. PMID: 28811319
  3. these data suggest that Gal-8 promotes the differentiation of highly suppressive Treg cells, which has implications for the treatment of inflammatory and autoimmune diseases. PMID: 26282995
  4. this study uncovers a unique molecular mechanism of lymphangiogenesis in which galectin-8-dependent crosstalk among VEGF-C, podoplanin and integrin pathways plays a key role. PMID: 27066737
  5. Gal-8mut was unable to induce T-cell proliferation, but efficiently co-stimulated antigen-specific responses, bothin vitroandin vivo. PMID: 26795039
  6. Gal-8 emerges as an attractive therapeutic candidate. PMID: 26126176
  7. Gal-8 stimulated naive T cells, but had an antiproliferative effect when these cells were activated.A dose of recombinant Gal-8 administered with a suboptimal Ag dose to DO11.10 mice strengthened weak responses in vivo. PMID: 22357632
  8. a novel role for Gal-8 in collaboration with Gal-1 in plasma cell formation, and suggest the possibility of using distinct LacNAc ligands to modulate the function of Gals. PMID: 21753146
  9. The findings of the present study delineate the differential molecular requirements for Gal-8 activities on T-cells, and suggest a dual activity relying on activation state. PMID: 21091438
  10. galectin-8 is expressed in the thymus in mouse, which suggests an active role for this lectin in shaping the mature T cell repertoire PMID: 17893094
  11. Taken together, our findings support a distinctive role for locally produced Gal-8 as an enhancer of otherwise borderline immune responses and also suggest that Gal-8 might fuel the reactivity at inflammatory foci. PMID: 19401394

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Subcellular Location
Cytoplasmic vesicle. Cytoplasm, cytosol.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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