Recombinant Mouse Golgi resident protein GCP60 (Acbd3)

Code CSB-YP804833MO
MSDS
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Source Yeast
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Code CSB-EP804833MO
MSDS
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Source E.coli
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Code CSB-EP804833MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP804833MO
MSDS
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Source Baculovirus
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Code CSB-MP804833MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Acbd3
Uniprot No.
Alternative Names
Acbd3; Gcp60; Pap7; Golgi resident protein GCP60; Acyl-CoA-binding domain-containing protein 3; Golgi complex-associated protein 1; GOCAP1; Golgi phosphoprotein 1; GOLPH1; PBR- and PKA-associated protein 7; Peripheral benzodiazepine receptor-associated protein PAP7
Species
Mus musculus (Mouse)
Expression Region
2-525
Target Protein Sequence
AAQLNVEQL EVSLDGLTLS PDSEERPGAE GAPPQTPPSS APGNGLGSGA SGQQREPGEA AAEGAAEEAR RMEQHWGFGL EELYGLALRF YKIKDGKAFH PTYEEKLKFV ALHKQVLLGP YNPDTSPEVG FFDVLGNDRR REWAALGNMS KEDAMVEFVK LLNKCCPLLS AYVASHRIEK EEEEKRRKAE EERRQREEEE RERLQKEEEK RKREKEDRLR REEEERRRIE EERLRLEQQK QQIMAALNSQ TAVQFQQYAA QQYPGNYEQQ QILIRQLQEQ HYQQYMQQLY QVQLAQQQAA LQKQQEVVMA GASLPASSKV NTAGASDTLS VNGQAKTHTE NSEKVLEPEA AEEALENGPK DSLPVIAAPS MWTRPQIKDF KEKIRQDADS VITVRRGEVV TVRVPTHEEG SYLFWEFATD SYDIGFGVYF EWTDSPNAAV SVHVSESSDE EEEEEENVTC EEKAKKNANK PLLDEIVPVY RRDCHEEVYA GSHQYPGRGV YLLKFDNSYS LWRSKSVYYR VYYTR
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells. Recruits PI4KB to the Golgi apparatus membrane; enhances the enzyme activity of PI4KB activity via its membrane recruitment thereby increasing the local concentration of the substrate in the vicinity of the kinase.
Gene References into Functions
  1. these findings suggest that ACBD3 has prominent impacts on cellular NAD(+) metabolism via regulating PARP1 activation-dependent auto-modification and thus cell metabolism and function. PMID: 25940138
  2. These results suggest that PBR, in addition to its role in cholesterol and coproporphyrinogen III transport, is also directing the mitochondrial PPIX import, a function that can be ascribed to the 18kDa PBR protein alone PMID: 14623258
  3. Study reports that ACBD3 is a Numb partner in cell-fate specification, ACBD3 and Numb proteins interact through an essential Numb domain, and the respective loss- and gain-of-function mutant mice share phenotypic similarities. PMID: 17418793
Subcellular Location
Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion. Note=Also mitochondrial (via its interaction with PBR).
Tissue Specificity
Expressed in brain (hippocampus, olfactory bulb, neuronal and glial cells of the cortex), eye, submaxillary gland, testis (interstitial and tubular compartments), ovary (granulosa cells, theca cells at late stages and primary follicles), adrenal gland (fa
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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