Recombinant Mouse HORMA domain-containing protein 1 (Hormad1)

Code CSB-YP863520MO
MSDS
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Source Yeast
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Code CSB-EP863520MO
MSDS
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Source E.coli
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Code CSB-EP863520MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863520MO
MSDS
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Source Baculovirus
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Code CSB-MP863520MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Hormad1
Uniprot No.
Alternative Names
Hormad1; Nohma; HORMA domain-containing protein 1; Newborn ovary HORMA protein
Species
Mus musculus (Mouse)
Expression Region
1-392
Target Protein Sequence
MATMQLQRTA SLSALVFPNK ISTEHQSLMF VKRLLAVSVS CITYLRGIFP ERAYGTRYLD DLCVKILKED KNCPGSSQLV KWMLGCYDAL QKKYLRMIIL AVYTNPGDPQ TISECYQFKF KYTKNGPIMD FISKNQNNKS STTSADTKKA SILLIRKIYV LMQNLGPLPN DVCLTMKLFY YDEVTPPDYQ PPGFKDGDCE GVIFDGDPTY LNVGEVPTPF HTFRLKVTTE KERMENIDST ILKPKESKTQ FEKILMDKDD VEDENHNNFD IKTKMNEQNE NSGASEIKEP NLDCKEEETM QFKKSQSPSI SHCQVEQLVS KTSELDVSES KTRSGKIFQS KMVNGNNQQG QTSKENRKRS LRQFRKTVLH VLESSQESVL KKRRVSEPKE HT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a key role in meiotic progression. Regulates 3 different functions during meiosis: ensures that sufficient numbers of processed DNA double-strand breaks (DSBs) are available for successful homology search by increasing the steady-state numbers of single-stranded DSB ends. Promotes synaptonemal-complex formation independently of its role in homology search. Plays a key role in the male mid-pachytene checkpoint and the female meiotic prophase checkpoint: required for efficient build-up of ATR activity on unsynapsed chromosome regions, a process believed to form the basis of meiotic silencing of unsynapsed chromatin (MSUC) and meiotic prophase quality control in both sexes.
Gene References into Functions
  1. Exogenous DSBs recruit the meiosis-specific homologous recombination repair machinery, are translocated to the axial elements, contribute to homology recognition, and that their repair is modulated by HORMAD1. PMID: 29414051
  2. that HORMAD1-IHO1 interaction provides a mechanism for the selective promotion of DNA double-strand break formation along unsynapsed chromosome axes PMID: 27723721
  3. These results indicate that Hormad1 deficiency promotes DMC1-independent double-strand break repairs, which in turn helps asynaptic Hormad1(-/-) oocytes resist perinatal loss. PMID: 23759310
  4. By the analysis of Hormad1/Spo11 double mutants, the Hormad1 deficiency was found to abrogate the massive oocyte loss in the Spo11-deficient ovary. The Hormad1 deficiency also causes the eventual loss of pseudo sex body in the Spo11-deficient ovary and testis. PMID: 22530760
  5. propose that the synaptonemal complex and HORMAD1 are key components of a negative feedback loop that coordinates meiotic progression with homologue alignment: HORMAD1 promotes homologue alignment and synaptonemal-complex formation PMID: 21478856
  6. HORMAD1 is a critical component of the synaptonemal complex that affects synapsis, recombination, and meiotic sex chromosome inactivation and transcriptional silencing. PMID: 21079677
  7. HORMAD1 and HORMAD2 are depleted from synapsed chromosome axes with the help of TRIP13. PMID: 19851446
  8. Results suggest that also mammalian cells use a HORMA domain-containing protein (HORMAD1) as part of a surveillance system that monitors chromosomal synapsis or other interactions between homologs. PMID: 19686734
  9. The expression pattern of Nohma suggests that it may be a critical regulatory protein in germ cell meiosis. PMID: 15567723

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Subcellular Location
[Isoform 1]: Nucleus. Chromosome.; [Isoform 2]: Cytoplasm.
Tissue Specificity
Specifically expressed in meiotic germ cells.
Database Links
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