Recombinant Mouse Helix-loop-helix protein 1 (Nhlh1)

Code CSB-YP015788MO
MSDS
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Source Yeast
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Code CSB-EP015788MO
MSDS
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Source E.coli
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Code CSB-EP015788MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015788MO
MSDS
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Source Baculovirus
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Code CSB-MP015788MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nhlh1
Uniprot No.
Alternative Names
Nhlh1; Hen1; Helix-loop-helix protein 1; HEN-1; Nescient helix loop helix 1; NSCL-1
Species
Mus musculus (Mouse)
Expression Region
1-133
Target Protein Sequence
MMLNSDTMEL DLPPTHSETE SGFSDCGGGP GPDGAGSGDP GVVQVRSSEL GESGRKDLQH LSREERRRRR RATAKYRTAH ATRERIRVEA FNLAFAELRK LLPTLPPDKK LSKIEILRLA ICYISYLNHV LDV
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May serve as DNA-binding protein and may be involved in the control of cell-type determination, possibly within the developing nervous system.
Gene References into Functions
  1. Predisposition to arrhythmia and autonomic dysfunction in Nhlh1-deficient mice. PMID: 12077327
  2. expression in the olfactory epithelium (OE) and the vomeronasal organ (VNO) during development; may function during development rather than in the adult stage PMID: 14578122
  3. We conclude that NSCL-1/2 maintain migration and survival of cells in the migratory stream to the cerebellum. PMID: 17573818
  4. The mouse homolog of HEN1 is expressed specifically in testis and methylates 3' termini of Piwi-interacting RNAs in vitro. PMID: 17652135
  5. Insm1 and Nhlh1/NSCL1 are novel HH targets induced by Shh treatment in cultured cerebellar granule cell progenitors. PMID: 18231642

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Subcellular Location
Nucleus.
Database Links
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