Recombinant Mouse High mobility group nucleosome-binding domain-containing protein 5 (Hmgn5)

Code CSB-YP864362MO
MSDS
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Source Yeast
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Code CSB-EP864362MO
MSDS
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Source E.coli
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Code CSB-EP864362MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP864362MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP864362MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Hmgn5
Uniprot No.
Alternative Names
Hmgn5; Garp45; Nsbp1; High mobility group nucleosome-binding domain-containing protein 5; Nucleosome-binding protein 1; Nucleosome-binding protein 45; NBP-45; Protein GARP45
Species
Mus musculus (Mouse)
Expression Region
1-406
Target Protein Sequence
MPKRKAAGDV SQEPKRRSAR LSAMPVPFTP ELKPKRASTS RKTKTTNVVE ENKDASTIPI PETKPEDVKD ECNMENAENG EAKIMEAPIP KMEAEEVKEQ INEDTEEDGG EKKEAVAAEA KDDELKANIQ DVEKDEDGKE HKDTGEEVED GKIEEEGLNE KPGTAKSEDA EVSKDEEEKG DNEKGEDGKE EGDEKEEEKD DKEGDTGTEK EVKEQNKEAE EDDGKCKEEE NKEVGKEGQP EEDGKEDLHE EVGKEDLHEE DGKEGQPEED GKEIHHEEDG KEGQPEEDGK EYLHEEDGEE GQPKEDQKEG QPEEDGKEDQ PEEDGKEGQC KEDGKEGHHE EGGKEDLHEE DGKEKDGGKE DRKEEGEQEV AVDEGSDENK VEAEEEGAEN KDFKQDGEKE EPLSIV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction.
Gene References into Functions
  1. Hmgn5 might act downstream of Hoxa10 to regulate the expression of Cox-2, Vegf and Mmp2. PMID: 27579887
  2. Mice overexpressing HMGN5, either globally or only in the heart, are normal at birth but develop hypertrophic heart with large cardiomyoctyes, deformed nuclei and disrupted lamina, and die of cardiac malfunction. PMID: 25609380
  3. HMGN5 overexpression induces neurite outgrowth. PMID: 25825524
  4. functional loss of HMGN5 leads to changes in transcription of Gpx6 and Hk1 that alter glutathione metabolism. PMID: 24392144
  5. Phenotypic analysis of Hmgn1(tm1/tm1), Hmgn3(tm1/tm1), and Hmgn5(tm1/tm1) mice and their wild type littermates with a battery of standardized tests uncovered variant-specific abnormalities. PMID: 23620591
  6. Downregulation of NSBP1 reduced the cancer growth rate, whereas tumourigenicity was not influenced. PMID: 20531280
  7. NSBP1 is an architectural protein that binds preferentially to euchromatin and modulates the fidelity of the cellular transcription profile by counteracting the chromatin-condensing activity of linker histones. PMID: 19748358

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Subcellular Location
Nucleus. Note=Associates with nucleosomes in euchromatin and is largely excluded from constitutive heterochromatin.
Protein Families
HMGN family
Tissue Specificity
Expressed in liver, spleen, lung, heart, kidney, muscle and brain (at protein level). Widely expressed with highest levels in submaxillary gland, thymus, kidney and liver and lowest levels in brain, lung, pancreas and eye.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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