Recombinant Mouse Homeobox protein MIXL1 (Mixl1)

Code CSB-YP895286MO
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP895286MO
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP895286MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP895286MO
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP895286MO
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mixl1
Uniprot No.
Alternative Names
Mixl1; Mix; MmlHomeobox protein MIXL1; Homeodomain protein MIX; mMix; MIX1 homeobox-like protein 1; Mix.1 homeobox-like protein
Species
Mus musculus (Mouse)
Expression Region
1-231
Target Protein Sequence
MAAAGSQQLQ FAEGAAFPIF PAAHPGGQLL PAMRPASGLP AAPHDSRAPA ATQCFPNRDS SPTAQTPAGL DPPGPSKGSA APSAPQRRKR TSFSSEQLQL LELVFRQTMY PDIHLRERLA ALTLLPESRI QVWFQNRRAK SRRQSGKSFQ PLSSRRGVFL HCPAPGTEAR CLKPQLPLEA DVNHVPDPSM TGGGVCTSGS QSFETYSSLS EDIGSKLDSW EEHIFSALGN F
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression.
Gene References into Functions
  1. In this study, we demonstrated that forced expression of Mix-like protein 1 (encoded by Mixl1) can be used to guide contribution of mouse embryonic stem cells to endodermal organs after blastocyst injection. PMID: 25192056
  2. Embryonic localization of Mixl1 shows its co-localized with the early hematopoietic marker, Runx1, in the allantois and visceral yolk sac blood islands. PMID: 24632399
  3. At later stages, in contrast with wild-type embryos, E7.5-8.0, Mixl1 knockout (KO) KO/KO embryos lacked morphologically recognizable midline structures such as the node and notochord. PMID: 24596343
  4. These data support the hypothesis that Mixl1 directly regulates Pdgfralpha and Flk1 gene expression. PMID: 22265737
  5. results strongly suggest that Gsc is a direct target gene of Mixl1 during embryogenesis. PMID: 19711456
  6. required for the morphogenesis of axial mesoderm, the heart and the gut during embryogenesis PMID: 12117810
  7. non-cell autonomous role in nascent mesoderm in the formation and/or patterning of adjacent definitive endoderm. PMID: 12619131
  8. Mixl1 is required for efficient haematopoiesis and BMP4-induced ventral mesoderm patterning in differentiating ES cells PMID: 15673572
  9. all mice reconstituted with Mixl1-transduced bone marrow developed fatal, transplantable acute myeloid leukemia with a mean latency period of 200 days PMID: 17060613
  10. As goosecoid is itself induced in a Foxh1-dependent manner, we propose that Foxh1 initiates positive and negative transcriptional circuits to refine cell fate decisions during gastrulation PMID: 17568773
  11. Overexpression of Mixl1 appears capable of inducing an abnormal self-renewal capacity in granulocytic precursors PMID: 18056627
  12. The studies suggest that high levels of Mixl1 preferentially allocate cells to the endoderm during ESC differentiation. PMID: 19038793

Show More

Hide All

Subcellular Location
Nucleus.
Protein Families
Paired homeobox family
Tissue Specificity
Expressed in the primitive streak of the gastrulating embryo, and marks cells destined to form mesoderm and endoderm. Present in differentiating embryonic stem cells (at protein level).
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1