Recombinant Mouse Homeobox protein aristaless-like 4 (Alx4)

Code CSB-YP001647MO
MSDS
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Source Yeast
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Code CSB-EP001647MO
MSDS
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Source E.coli
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Code CSB-EP001647MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001647MO
MSDS
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Source Baculovirus
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Code CSB-MP001647MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Alx4
Uniprot No.
Alternative Names
Alx4Homeobox protein aristaless-like 4; ALX-4
Species
Mus musculus (Mouse)
Expression Region
1-399
Target Protein Sequence
MNAETCVSYC ESPAAAMDAY YSPVSQSREG SSPFRGFPGG DKFGTTFLSA GAKGQGFGDA KSRARYGAGQ QDLAAPLESS SGARGSFNKF QPQPPTPQPP PAPPAPPAHL YLQRGACKTP PDGSLKLQEG SGGHNAALQV PCYAKESNLG EPELPPDSEP VGMDNSYLSV KETGAKGPQD RASAEIPSPL EKTDSESNKG KKRRNRTTFT SYQLEELEKV FQKTHYPDVY AREQLAMRTD LTEARVQVWF QNRRAKWRKR ERFGQMQQVR THFSTAYELP LLTRAENYAQ IQNPSWIGNN GAASPVPACV VPCDPVPACM SPHAHPPGSG ASSVSDFLSV SGAGSHVGQT HMGSLFGAAG ISPGLNGYEM NGEPDRKTSS IAALRMKAKE HSAAISWAT
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor involved in skull and limb development.
Gene References into Functions
  1. Inactivation of ALX4/Alx4 causes lacrimal gland aplasia in both human and mouse. These results reveal a key role of Alx4 in mediating FGF-Shp2-FGF signaling in the neural crest for lacrimal gland development. PMID: 29028795
  2. novel allele of Alx4 results in reduced Fgf10 expression and failure of eyelid fusion in mice PMID: 25673119
  3. Alx4 genetically interacts with and Shh and Gli3 during genital tubercle formation. PMID: 23942202
  4. A mutagenesis study characterizes a polydactylous phenotype that is caused by a nonsense mutation in the Alx4 gene. PMID: 23979902
  5. Progression of calvarial bone development requires Foxc1 regulation of Msx2 and Alx4. PMID: 14512019
  6. the loss of the severe preaxial polydactyly characteristic of Gli3-/- limbs in double mutant embryos establishes that this type of polydactyly requires Alx4 function. PMID: 15968591
  7. Function and regulation of Alx4 in limb development entail complex genetic interactions with Gli3 and Shh. PMID: 16039644
  8. Alx4, is required for normal branching morphogenesis of the ductal epithelia during pubescent mammary gland development. PMID: 16916507

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Involvement in disease
Defects in Alx4 are the cause of Strong luxoid (lst) phenotype. At heterozygosity lst is characterized by preaxial abnormalities of the hindfeet and, very rarely, of the forefeet. Homozygotes show preaxial polydactyly of all four limbs, reductions and duplications of the radius, absence of the tibia, craniofacial defects, reduction of the pubis, and dorsal alopecia.
Subcellular Location
Nucleus.
Protein Families
Paired homeobox family
Tissue Specificity
Expressed in osteoblasts. Not expressed in brain, heart, intestine, kidney, liver, muscle, spleen and testis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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