Recombinant Mouse Homeobox protein orthopedia (Otp)

Code CSB-YP017279MO
MSDS
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Source Yeast
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Code CSB-EP017279MO
MSDS
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Source E.coli
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Code CSB-EP017279MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017279MO
MSDS
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Source Baculovirus
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Code CSB-MP017279MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Otp
Uniprot No.
Alternative Names
OtpHomeobox protein orthopedia
Species
Mus musculus (Mouse)
Expression Region
1-325
Target Protein Sequence
MLSHADLLDA RLGMKDAAEL LGHREAVKCR LGVGGSDPGG HPGDLAPNSD PVEGATLLPG EDITTVGSTP ASLAVSAKDP DKQPGPQGGP NPSQAGQQQG QQKQKRHRTR FTPAQLNELE RSFAKTHYPD IFMREELALR IGLTESRVQV WFQNRRAKWK KRKKTTNVFR APGTLLPTPG LPQFPSAAAA AAAAMGDSLC SFHANDTRWA AAAMPGVSQL PLPPALGRQQ AMAQSLSQCS LAAGPPPNSM GLSNSLAGSN GAGLQSHLYQ PAFPGMVPAS LPGPSNVSGS PQLCSSPDSS DVWRGTSIAS LRRKALEHTV SMSFT
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the specification of hypothalamic neuroendocrine cells. Specifically required for the specification of diencephalic dopaminergic neurons of the A11 group.
Gene References into Functions
  1. Studies revealed that Dlx1/2 and Otp are required for the specification of growth hormone-releasing hormone (GHRH)- and AgRP-neurons, respectively. Dlx1/2 suppress agouti-related protein (AgRP)-neuronal fate by directly binding and repressing the Otp gene, uncovering a novel Dlx1/2-Otp gene regulatory axis critical for the segregation of GHRH- and AgRP-neuronal fates. PMID: 29795232
  2. Otp and TTF-1 are essential for the normal morphological development of the hypothalamus, including the arcuate nucleus [review] PMID: 23276958
  3. diencephalic neurons colonize the telencephalic amygdaloid complex, migrating along a tangential route that crosses a boundary between developing brain vesicles. The diencephalic transcription factor OTP was necessary for this migratory behavior PMID: 20495559
Subcellular Location
Nucleus.
Protein Families
Paired homeobox family, Bicoid subfamily
Tissue Specificity
Restricted regions of the developing forebrain, hindbrain, and spinal cord.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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