Recombinant Mouse Interferon-induced protein 44-like(IFI44L)

Code CSB-YP011015MO
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Source Yeast
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Code CSB-EP011015MO
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Source E.coli
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Code CSB-EP011015MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011015MO
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Source Baculovirus
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Code CSB-MP011015MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Ifi44l
Uniprot No. Q9BDB7
Alternative Names Ifi44l; H28Interferon-induced protein 44-like [Cleaved into: Minor histocompatibility antigen HA-28; HLA-HA28; IFL8)]
Species Mus musculus (Mouse)
Expression Region 1-447
Target Protein Sequence MKVTARLTWI EEKILEKLLG NASLTLLYQS SAHKNCVSEM TQKYSLQGST MTVFHLEKDV VVGVFILENF PRLVSEKPCT CAWFSLKRNN SSGISALFLN TKVIVDSEEL IIFSLDGLSL SVTPLRGFTL ALNDTVMNGL ELNLGHGFLP VECEIFRVDG IKKNPSFIKK MVTAEQHRGK LLSALRAYKP YKDLVSEVRI LLVGPVGSGK SSFFNSVKSA FQGHLTRQAI VGSDESSITK QYRVYSIKDG KSGETLPFML CDSMGLEEGE EAGLCIDDIP HILQGCVPDR YQFNPCEPMK PKHSPHAASP PLKDRIHCVA FVLHINSVNT LSDKMVAKLK KIRKDVVDCG IGYVALLTNV EEYDEVLDDS FANMTETVTS LSQVQNVQKW LNIPIANILM VSNYASERRL EPMKDILVFA ALRQMLRAAD DALEDLPLED TGNLAPF
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Precursor of the histocompatibility antigen HA-28 in BALB.B mice. More generally, minor histocompatibility antigens refer to immunogenic peptide which, when complexed with MHC, can generate an immune response after recognition by specific T-cells. The peptides are derived from polymorphic intracellular proteins, which are cleaved by normal pathways of antigen processing. The binding of these peptides to MHC molecules and its expression on the cell surface can stimulate T-cell responses and thereby trigger graft rejection or graft-versus-host disease (GVHD). More specifically, HA-28 minor antigen is transcribed in the BALB.B donor but not in host C57BL/6 cells. HA-28 is presented to the donor BALB.B cell surface by Kb MHC. This complex HA-28/Kb MHC elicits cytotoxic T-cell response in C57BL/6 mice immunized with BALB.B spleen cells. It induces C57BL/6 mice cells recognition and lysis by CD8 T-cell from BALB.B mice.
Gene References into Functions
  1. Data identified Tnni3k, Fpgt and H28 as likely likely candidates for Vms1. PMID: 21525387
Subcellular Location Cytoplasm.
Protein Families IFI44 family
Tissue Specificity Expressed on cells of the hematopoietic lineage. Detected in transformed cell lines of the macrophage and B-cell lineage. Expressed in spleen and bone marrow.
Database Links

KEGG: mmu:15061

STRING: 10090.ENSMUSP00000044765

UniGene: Mm.219544

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