Recombinant Mouse Kelch repeat and BTB domain-containing protein 5 (Kbtbd5)

Code CSB-YP875223MO
MSDS
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Source Yeast
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Code CSB-EP875223MO
MSDS
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Source E.coli
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Code CSB-EP875223MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP875223MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP875223MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Klhl40
Uniprot No.
Alternative Names
Klhl40; Kbtbd5; Kelch-like protein 40; Kelch repeat and BTB domain-containing protein 5
Species
Mus musculus (Mouse)
Expression Region
1-621
Target Protein Sequence
MTLGLEQAEE QRLYQQTLLQ DGLKDMLDHG KFLDCVVRVG EREFPCHRLV LAACSPYFRA RFLAEPDSAG EVRLEEVSPD VVSQVLHYLY TSEIALDEAS VQDLFAAAHR FQIPSIFTIC VSFLQKRLCL ANCLAVFRLG LLLDCARLAV AARDFICARF PLVARDNDFL GLSADELIAI ISSDGLNVEK EEAVFEAVMR WASSGDAEAQ AERQRALPTV FESVRCRLLP RAFLETRVER HPLVRSQPEL LRKVQMVKDA HEGRLTTLRK KKKEKGEQTA RAKEANQGTE DTKAEDDEER VLPGILNDTL RFGMFLQDLI FMISEEGAVA YDPAANECYC ASLSTQIPKN HVSLVTKENQ VFVAGGLFYN EDNKEDPMSA YFLQFDHLDS EWLGMPPLPS PRCLFGLGEA LNAIYVVGGR ELKDSEDSLD SVLCYDRLSF KWGESDPLPY AVYGHTVLSH MDLVYVIGGK GKDRKCLNKM CVYDPKKFEW KELAPMQTAR SLFGATVHDG RIFVAAGVTD TGLTSSSEVY SIADNKWTSF EAFPQERSSL SLVSLAGTLY ALGGFATLET ESGELVPTEL NDIWRYNEDE KKWEGVLREI AYAAGATFLP VRLNVVRLTK M
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a key regulator of skeletal muscle development. The BCR(KLHL40) complex acts by mediating ubiquitination and degradation of TFDP1, thereby regulating the activity of the E2F:DP transcription factor complex. Promotes stabilization of LMOD3 by acting as a negative regulator of LMOD3 ubiquitination; the molecular process by which it negatively regulates ubiquitination of LMOD3 is however unclear.
Gene References into Functions
  1. Kbtbd5 regulates skeletal muscle myogenesis through the regulation of E2F1-DP1 activity PMID: 25940086
  2. NEB and LMOD3 were reduced in skeletal muscle of Klhl40-/- mice, providing a potential basis for the development of experimental nemaline myopathy. PMID: 24960163
  3. we have demonstrated that Kbtbd5 is the direct downstream target gene of the Myod family and regulates myogenic differentiation. PMID: 24361185
Subcellular Location
Cytoplasm. Cytoplasm, myofibril, sarcomere, A band. Cytoplasm, myofibril, sarcomere, I band.
Protein Families
KLHL40 family
Tissue Specificity
Specifically expressed in skeletal muscles in embryonic, neonatal and adults. Expressed in various types of muscles, including extensor digitorum longus, gastrocnemius, soleus, diaphragm, masseter and heart (at protein level). Not detected in brain, liver
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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