Recombinant Mouse Krueppel-like factor 16 (Klf16)

Code CSB-YP012390MO
MSDS
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Source Yeast
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Code CSB-EP012390MO
MSDS
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Source E.coli
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Code CSB-EP012390MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP012390MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP012390MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Klf16
Uniprot No.
Alternative Names
Klf16; Bteb4; DrrfKrueppel-like factor 16; Basic transcription element-binding protein 4; BTE-binding protein 4; Dopamine receptor-regulating factor; Transcription factor BTEB4
Species
Mus musculus (Mouse)
Expression Region
1-251
Target Protein Sequence
MSAAVACVDY FAADVLMAIS SGAVVHRGRP GPEGAGPAAG LDVRATRREA TPPGTPGAPP PPATAPGPGG ATAAPHLLAA SILADLRGGP VVATAASTAG GTSPVSSSSA ASSPSSGRAP GAAKSHRCPF HGCAKAYYKS SHLKSHLRTH TGERPFACDW PGCDKKFARS DELARHHRTH TGEKRFPCPL CTKRFTRSDH LTKHARRHPG FRPELLRRPG ARSVSPSDSL PCSLAGSPTP SPVPSPAPAG L
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that binds GC and GT boxes in the D1A, D2 and D3 dopamine receptor promoters and displaces Sp1 and Sp3 from these sequences. It modulates dopaminergic transmission in the brain by repressing or activating transcription from several different promoters depending on cellular context.
Gene References into Functions
  1. results indicate that KLF16 inhibits adipogenesis through downregulation of PPARgamma expression PMID: 27611969
  2. methylation of only six of 98 CpG dinucleotides within the EphA5 promoter blocks its transactivation by KLF16 but enables transactivation by KLF2 and KLF15. PMID: 27402841
  3. DRRF mRNA is expressed uniquely during development at all time points tested with high levels observed at E12, E14 and E16 in various tissues PMID: 11744383
  4. DRRF auto-regulates its own promoter PMID: 18472212
Subcellular Location
Nucleus.
Protein Families
Sp1 C2H2-type zinc-finger protein family
Tissue Specificity
High expression in brain; olfactory tubercle, olfactory bulb, nucleus accumbens, striatum, hippocampal CA1 region, amygdala, dentate gyrus and frontal cortex. Moderate expression in hippocampal CA2-3 regions, piriform cortex, septum, and distinct thalamic
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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