Recombinant Mouse LDLR chaperone MESD (Mesdc2)

Code CSB-YP880641MO
MSDS
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Source Yeast
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Code CSB-EP880641MO
MSDS
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Source E.coli
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Code CSB-EP880641MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880641MO
MSDS
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Source Baculovirus
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Code CSB-MP880641MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mesd
Uniprot No.
Alternative Names
Mesd; Mesdc2LRP chaperone MESD; LDLR chaperone MESD; Mesoderm development candidate 2; Mesoderm development protein
Species
Mus musculus (Mouse)
Expression Region
30-224
Target Protein Sequence
A DTPGEATPPP RKKKDIRDYN DADMARLLEQ WEKDDDIEEG DLPEHKRPSA PIDFSKLDPG KPESILKMTK KGKTLMMFVT VSGNPTEKET EEITSLWQGS LFNANYDVQR FIVGSDRAIF MLRDGSYAWE IKDFLVSQDR CAEVTLEGQM YPGKGGGSKE KNKTKPEKAK KKEGDPKPRA SKEDNRAGSR REDL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway through chaperoning the coreceptors of the canonical Wnt pathway, LRP5 and LRP6, to the plasma membrane. Essential for specification of embryonic polarity and mesoderm induction. Plays an essential role in neuromuscular junction (NMJ) formation by promoting cell-surface expression of LRP4. May regulate phagocytosis of apoptotic retinal pigment epithelium (RPE) cells.
Gene References into Functions
  1. Mescd2 binds to Lrp4 and regulates Lrp4 surface expression in myotubes. PMID: 24140340
  2. Mesd C-terminal region constitutes the major LRP5/6-binding domain, and that Mesd protein and its C-terminal region peptide have a potential therapeutic value in cancer PMID: 23469146
  3. The region 45-184 of MESD is essential and sufficient to act as a molecular chaperone for the low-density lipoprotein receptor (LDLR) family. PMID: 21397185
  4. We show that MESD contains a central folded domain flanked by unstructured regions required to facilitate maturation of LRP6. Enforced expression of human MESD promotes the secretion of soluble minireceptors derived from LRP6. PMID: 17488095
  5. Functional analyses of the Lrp6 rs mutant showed decreased targeting to plasma membrane because of reduced interaction with Mesoderm development (Mesd), a chaperone for Lrp6, leading to impaired Wnt/beta-catenin signaling PMID: 18505367
  6. Secondary structure prediction using chemical shift data indicates that MESD(12-155) contains four a-helices and five b-sheets. PMID: 19636811
  7. Mesd not only blocks binding of Dickkopf (DKK)-1 and sclerostin proteins to low-density lipoprotein receptor-related protein (LRP)5 and LRP6 but also inhibits Wnt/beta-catenin signaling in LRP5- or LRP6- expressing cancer cells. PMID: 20446724
  8. Together our results show that in addition to serving as a folding chaperone, Mesd can function as a receptor antagonist by inhibiting ligand binding to mature LRP6. PMID: 16263759

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Subcellular Location
Endoplasmic reticulum.
Protein Families
MESD family
Tissue Specificity
Expressed in many tissues, but not in skeletal muscles. In the retina expressed in retinal ganglion cells, inner and outer plexiform layers, photoreceptor inner and outer segments and retinal pigment epithelium (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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