Recombinant Mouse Leucine-rich repeat and WD repeat-containing protein 1 (LRWD1)

Code CSB-YP805961MO
MSDS
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Source Yeast
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Code CSB-EP805961MO
MSDS
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Source E.coli
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Code CSB-EP805961MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP805961MO
MSDS
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Source Baculovirus
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Code CSB-MP805961MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LRWD1
Uniprot No.
Alternative Names
LRWD1; OrcaLeucine-rich repeat and WD repeat-containing protein 1; ORC-associated protein; ORCA; Origin recognition complex-associated protein
Species
Mus musculus (Mouse)
Expression Region
1-648
Target Protein Sequence
MAPLTPQLLL QRGRPKTDKL GKIQSLNLSG LQLLSEHLDP NLLGRLKKLR ELDLSNNLLE TLPANLGLSH LRILRCTNNQ LGDVTALHQF PELEELNLEG NPFLTVSDNL KVSFLLPKLR KVNGKDTAST CSQVENLDRE LMDRVTAHWQ KFIATVSPEE ETDKVRADFM RSAVRDVCYG PESLIEFTQW RVRMIAEELV ASGGAQVQDA KVPVEHPQAA GASKFRAREV ASKRPGKDPV TLPPSKRVRA LPPAQAEGSP MGADGGQAAL HLEPLHFLQC HSRNNSPKDL ETQLWACAFE PAREEGHSRA TSQTVATCGG EAVCVIDCQT GLVLHKYKVP GEEFFSVAWT ALTVVTQAGH KKRWNMLAAA GLRGMVRLLH VRAGFCCSVI RAHKKAIATL CFSPSHETHL FTASYDKRII LWDIGVPNQD YKFQASQLLT LNCGSVPLRL CPVATCPDDF LLAGCEGGCY CWDVRLDQPQ KQRVCEVNFI FSEDSKVSGQ RVDGLAFVNE DVVASKGSGQ GTIYLWSWSQ TWAGRGRQSV LPVVILVRLQ WSPTNLAYFS LSTCPGKNLV LCGDEEGSVW IYDVEHLLKE PPQATTLQPP TQILKWPQPT ALGQPVTKTM INTVVANAAF TYLTALTDSN IVSIWRRC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.
Gene References into Functions
  1. immunostaining and immunoprecipitation showed colocalization and interaction between lrwd1 and gamma-tubulin respectively, implicating lrwd1 as a candidate centrosomal protein. These results suggest that lrwd1 may play an important role in spermatogenesis. PMID: 20180869
  2. Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing PMID: 22427655
Subcellular Location
Nucleus. Chromosome, centromere. Chromosome, telomere. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore.
Protein Families
LRWD1 family
Tissue Specificity
Testis-specific.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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