Recombinant Mouse Leucine-rich repeat-containing protein 34 (Lrrc34)

Code CSB-YP861593MO
MSDS
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Source Yeast
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Code CSB-EP861593MO
MSDS
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Source E.coli
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Code CSB-EP861593MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861593MO
MSDS
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Source Baculovirus
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Code CSB-MP861593MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Lrrc34
Uniprot No.
Alternative Names
Lrrc34; Spata34Leucine-rich repeat-containing protein 34
Species
Mus musculus (Mouse)
Expression Region
1-415
Target Protein Sequence
MEKLQAQYCY LCSENIRKTN PFILNILQKL DEEIEKRPKE KFTVNIAGNN RLDSGQRITG EDFWLLSKTL RNQPCISGVD VRYNLIGDVG AFYAAKLLQK QPSITYLNLM FNDIGPEGGE LIAKALHKNK TLKYLRMTGN KIENTGGMLF AAMLQMNSSL EKLDLGDCDL GLQCVIAFST VLTQNQAIKG INLNRPILYG EQEESTVHIG HMLKENHVLV ELHMCKHGMK NYGLQQLCNA LYLNSSLRYL DVSCNKITRD GMVFLADVLK SNTTLEVLDL SFNRIETAGA KYLSETLTSH NRSLKALSVV SNKIEGEGLV ALSQSMKTNL VLSNIYIWGN KFDEDTCVAY SDLIKSGRLK PDNTDVEPYM VDEHIYLSEV SNGLKRHYYW APTYGETYMP SSSAGFALVP VGEHL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Highly expressed in stem cells where it may be involved in regulation of pluripotency. In embryonic stem cells (ESCs), important for normal expression of the pluripotency regulators POU5F1/OCT4 and KLF4. Also important for expression of the ectodermal marker gene NES and the endodermal marker gene GATA4. Promotes stem cell proliferation in vitro.
Gene References into Functions
  1. LRRC34 is a novel nucleolar protein that is predominantly expressed in pluripotent stem cells. PMID: 24991885
  2. Overexpression of Spata34 in COS7 cells inhibited the transcriptional activity of AP-1, p53 and p21 which suggested that Spata34 protein may act as a transcriptional repressor in p53 and p21 pathway PMID: 24435972
  3. Lrrc34 is most abundantly expressed in tissues rich in highly ciliated cells, such as olfactory sensory neurons, and is predicted to be important to cilia. PMID: 17971504
Subcellular Location
Nucleus. Nucleus, nucleolus. Cytoplasm.
Tissue Specificity
Expressed in testis where it specifically localizes to germ cells (at protein level). Not detected in other tissues tested (at protein level). Expressed in pluripotent embryonic stem cells and multipotent adult germline stem cells.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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