Recombinant Mouse Leupaxin (Lpxn)

Code CSB-YP013068MO
MSDS
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Source Yeast
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Code CSB-EP013068MO
MSDS
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Source E.coli
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Code CSB-EP013068MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013068MO
MSDS
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Source Baculovirus
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Code CSB-MP013068MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Lpxn
Uniprot No.
Alternative Names
Lpxn; Leupaxin
Species
Mus musculus (Mouse)
Expression Region
1-386
Target Protein Sequence
MEELDALLEE LERCTFQDSE EYSNPVSCHL DQQSTEESKI PQTPKTLSSQ GNTSPLKVQL VYATNIQEPN VYSEVQEPKE SVLPPKTSAA AQLDELMAHL SEMQAKVSVK ADTSRKPLPD QQDHKASLDS MLGDLEQELQ DLGIATVPKG YCASCQKPIA GKVIHALGQS WHPEHFVCTH CKEELGSSPF FERSGLAYCS KDYHRLFSPR CAYCAAPITD KVLTAMNKTW HPEHFFCSHC GEVFGAEGFH EKDKKPYCRK DFLAMFSPKC GGCNRPVLEN YLSAMNTVWH PECFVCGDCF SSFSSGSFFE LDGRPFCELH YHHRRGTLCH DCGQPITGRC ISAMGHKFHP EHFVCAFCLT QLPKGIFKEQ NNKTYCEKCF TKLFSQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional coactivator for androgen receptor (AR) and serum response factor (SRF). Contributes to the regulation of cell adhesion, spreading and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events. Suppresses the integrin-induced tyrosine phosphorylation of paxillin (PXN). May play a critical role as an adapter protein in the formation of the adhesion zone in osteoclasts. Negatively regulates B-cell antigen receptor (BCR) signaling.
Gene References into Functions
  1. leupaxin functions as a paxillin counterpart that potently suppresses the tyrosine phosphorylation of paxillin during integrin signaling. PMID: 19917054
  2. leupaxin may be a critical nucleating component of the osteoclast podosomal signaling complex PMID: 12674328
  3. Association of the scaffold protein LPXN with Src adds further complexity to the organization of the podosomal signaling complex in osteoclasts. PMID: 16914530
  4. Leupaxin plays an inhibitory role in BCR signaling and B cell function. PMID: 17640867
  5. Leupaxin is enriched in mouse vascular smooth muscle and its expression is dynamically regulated during development. PMID: 18497331

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Subcellular Location
Cytoplasm. Cell junction, focal adhesion. Nucleus. Cytoplasm, perinuclear region. Cell projection, podosome. Cell membrane.
Protein Families
Paxillin family
Tissue Specificity
Expressed in osteoclasts (at protein level). Highly expressed in vascular smooth muscle.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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