Recombinant Mouse Low-density lipoprotein receptor-related protein 2(Lrp2) ,partial

Code CSB-YP013096MO
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Source Yeast
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Code CSB-EP013096MO
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Source E.coli
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Code CSB-EP013096MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013096MO
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Source Baculovirus
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Code CSB-MP013096MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Lrp2
Uniprot No. A2ARV4
Alternative Names Lrp2Low-density lipoprotein receptor-related protein 2; LRP-2; Glycoprotein 330; gp330; Megalin
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Multiligand endocytic receptor. Acts together with CUBN to mediate endocytosis of high-density lipoproteins. Mediates receptor-mediated uptake of polybasic drugs such as aprotinin, aminoglycosides and polymyxin B. In the kidney, mediates the tubular uptake and clearance of leptin. Also mediates transport of leptin across the blood-brain barrier through endocytosis at the choroid plexus epithelium. Endocytosis of leptin in neuronal cells is required for hypothalamic leptin signaling and leptin-mediated regulation of feeding and body weight. Mediates endocytosis and subsequent lysosomal degradation of CST3 in kidney proximal tubule cells. Mediates renal uptake of 25-hydroxyvitamin D3 in complex with the vitamin D3 transporter GC/DBP. Mediates renal uptake of metallothionein-bound heavy metals. Together with CUBN, mediates renal reabsorption of myoglobin. Mediates renal uptake and subsequent lysosomal degradation of APOM. Plays a role in kidney selenium homeostasis by mediating renal endocytosis of selenoprotein SEPP1. Mediates renal uptake of the antiapoptotic protein BIRC5/survivin which may be important for functional integrity of the kidney. Mediates renal uptake of matrix metalloproteinase MMP2 in complex with metalloproteinase inhibitor TIMP1. Mediates endocytosis of Sonic hedgehog protein N-product (ShhN), the active product of SHH. Also mediates ShhN transcytosis. In the embryonic neuroepithelium, mediates endocytic uptake and degradation of BMP4, is required for correct SHH localization in the ventral neural tube and plays a role in patterning of the ventral telencephalon. Required at the onset of neurulation to sequester SHH on the apical surface of neuroepithelial cells of the rostral diencephalon ventral midline and to control PTCH1-dependent uptake and intracellular trafficking of SHH. During neurulation, required in neuroepithelial cells for uptake of folate bound to the folate receptor FOLR1 which is necessary for neural tube closure. In the adult brain, negatively regulates BMP signaling in the subependymal zone which enables neurogenesis to proceed. In astrocytes, mediates endocytosis of ALB which is required for the synthesis of the neurotrophic factor oleic acid. Involved in neurite branching. During optic nerve development, required for SHH-mediated migration and proliferation of oligodendrocyte precursor cells. Mediates endocytic uptake and clearance of SHH in the retinal margin which protects retinal progenitor cells from mitogenic stimuli and keeps them quiescent. Plays a role in reproductive organ development by mediating uptake in reproductive tissues of androgen and estrogen bound to the sex hormone binding protein SHBG. Mediates endocytosis of angiotensin-2. Also mediates endocytosis of angiotensin 1-7. Binds to the complex composed of beta-amyloid protein 40 and CLU/APOJ and mediates its endocytosis and lysosomal degradation. Required for embryonic heart development. Required for normal hearing, possibly through interaction with estrogen in the inner ear.
Gene References into Functions
  1. megalin is critical for mitochondrial biology; mitochondrial intracrine signaling is a continuum of the retrograde early endosome-to-Golgi-Rab32 pathway and defects in this pathway may underlie disease processes in many systems. PMID: 29916093
  2. TTR neuritogenic activity is mediated by the megalin receptor and is lost in megalin-deficient neurons. PMID: 27518433
  3. in a bleomycin-based model of acute lung injury, megalin(+/-) animals (the megalin(-/-) variant is lethal due to postnatal respiratory failure) showed a marked increase in intra-alveolar protein and more severe lung injury compared with wild-type littermates PMID: 28678521
  4. Cell type-specific glycoforms of megalin exist in the proximal tubular cells and modulate ligand absorption capacity. PMID: 27773703
  5. This study evaluated the role of transcobalamin (TC), the transcobalamin receptor (CD320) , and megalin in various tissues during pregnancy. PMID: 28351841
  6. miR-148b negatively regulates megalin expression in unilateral ureteral obstruction-induced kidney injury. PMID: 28331063
  7. Exocytosis-mediated urinary C-megalin excretion is associated with the development and progression of diabetic nephropathy in T2DM, particularly due to megalin-mediated lysosomal dysfunction in proximal tubules. PMID: 28289043
  8. LRP2 plays a crucial role in cardiovascular development in mice. PMID: 26822476
  9. Foxg1-Cre mediated Lrp2 inactivation in developing mouse neural retina, ciliary and retinal pigment epithelia is a model of congenital high myopia PMID: 26107939
  10. megalin-deficient mice may provide a valuable model for future studies of megalin in ocular physiology and pathology. PMID: 24980834
  11. LRP2 is essential for cellular folate uptake in the developing neural tube, a crucial step for proper neural tube closure. PMID: 24639464
  12. Multiligand endocytic receptor megalin may be involved in AT1a receptor-mediated uptake of angiotensin II by proximal tubule cells. PMID: 24740791
  13. SNPs in the CYP24A1, CYP2R1, calcium sensing receptor (CASR), vitamin D binding protein (GC), retinoid X receptor-alpha (RXRA) and megalin (LRP2) genes were significantly associated with pancreas cancer risk. PMID: 23826131
  14. Lrp2 is a novel target gene by progesterone and progesterone receptor. PMID: 24140060
  15. Data indicate that uptake of Sepp1 and Gpx3 by d-13 visceral yolk sac was independent of apoER2 and megalin. PMID: 23651543
  16. megalin mutation causes Donnai-Barrow/Facio-Oculo-Acustico-Renal syndrome. PMID: 23048173
  17. In the olfactory epithelium, megalin was found to be specifically expressed in sustentacular cells and the Megalin protein was located in their microvilli PMID: 21818577
  18. megalin might exhibit a proinflammatory effect by the uptake of retinoids in recruited monocytes, which thereby differentiate to liver macrophages and potentiate fibrogenesis by the release of proinflammatory mediators PMID: 21939757
  19. PPARalpha/gamma and their agonists positively control megalin expression.This regulation could have an important impact on pathophysiologies such as chronic kidney disease associated with diabetes and hypertension, in which megalin expression is impaired. PMID: 21311715
  20. Results provide evidence that MESD functions as a general LRP chaperone and suggest that the Mesd phenotype results from both signaling and endocytic defects resulting from misfolding of multiple LRP receptors. PMID: 21337463
  21. Megalin is involved in the renal reabsorption of radiolabelled octreotide, octreotate, exendin, neurotensin and minigastrin. PMID: 21170526
  22. megalin-dependent endocytosis has a role along the proximal nephron in the intrarenal renin angiotensin system PMID: 20966072
  23. Results confirm that megalin interacts with APP and FE65, suggesting that these three proteins form a tripartite complex. PMID: 20637285
  24. Mutation of megalin leads to urinary loss of selenoprotein P and selenium deficiency in serum, liver, kidneys and brain PMID: 20653565
  25. The progressive loss of megalin suggests that transport of metallothionein-I+II into the degenerating photoreceptors (from e.g., Muller cells), could be impaired in retinitis pigmentosa PMID: 20357188
  26. in ependymal cells, regulates adult neurogenesis PMID: 20460439
  27. is essential for renal proximal tubule reabsorption and accumulation of transcobalamin-B(12). PMID: 11832420
  28. Expression profiling confirms a crucial role for megalin in renal vitamin D metabolism. PMID: 12371967
  29. endocytic receptors megalin and cubilin are involved in renal reabsorption of myoglobin PMID: 12724130
  30. In RAP-deficient mice megalin expression was strongly reduced and restricted to a subapical localization and NaP(i)-IIa protein distribution and abundance in the kidney was not altered. PMID: 12748857
  31. megalin has a role in thyroid homeostasis with possible implications in thyroid diseases PMID: 14657389
  32. Megalin expression in the visceral ectoderm increases as embronic development progresses; strong expression of megalin is also observed in the neural ectoderm, neural plate and neural tube. PMID: 14983511
  33. results support a major role of megalin in thyroid hormone secretion PMID: 15878230
  34. the His-sRAP-induced acceleration of megalin-mediated endocytosis causes phosphaturia via altered subcellular distribution of NaPi-II [39-kD receptor-associated protein (RAP)] PMID: 15976002
  35. Megalin-mediated endocytosis in kidney proximal tubules prevents apoM excretion in the urine. PMID: 16099815
  36. The megalin, an endocytic receptor in reproductive tissues, acts as a pathway for cellular uptake of biologically active androgens and estrogens bound to sex hormone binding globulin(SHBG). PMID: 16143106
  37. These findings suggest that megalin has a crucial role in the development of astrocytes of the spinal cord. PMID: 16196028
  38. Megalin mediates IGF-I-induced clearance of Abeta and is involved in IGF-I transport into brain. Attenuated IGF-I/megalin input may contribute to increased risk of neurodegeneration, including late-onset Alzheimer's disease. PMID: 16306401
  39. Megalin in the ES lumen is a soluble component and may be endocytosed by the ES epithelial cells. Furthermore, we found that the tectorial membrane, an acellular structure in the cochlea, gave a strong megalin immunoreaction. PMID: 17063000
  40. enhanced protein endocytosis led to upregulation of profibrotic mediators in a megalin-dependent way; however, no evidence that endocytosis played a pathogenetic role in the development of the tubulointerstitial disease in magalin deficiency PMID: 17460141
  41. demonstrate that homozygous megalin mutant mice exhibit profound hearing loss at 3 months of age associated with features of presbycusis PMID: 17846082
  42. Dynamic expression of Lrp2 pathway members reveals progressive epithelial differentiation of primitive endoderm in mouse blastocyst. PMID: 18083160
  43. kidney selenium homeostasis is mediated by a megalin-dependent Sepp1 uptake pathway in the proximal tubule PMID: 18174160
  44. Megalin is essential in astro-oligodendroglial interactions during development of the spinal cord. PMID: 18388738
  45. Inactivation of RFC1 impacts the expression of several ligands and interacting proteins in the cubilin-amnionless-megalin complex that are involved in the maternal-fetal transport of folate and other nutrients. PMID: 18400109
  46. megalin plays a pivotal role in the reabsorption of small to large molecular size proteins and provides direct in vivo evidence that reabsorption of filtered proteins triggers events leading to tubule injury. PMID: 18769366
  47. The neuritogenic effect of transthyretin is dependent on megalin-mediated internalization during nerve regeneration. PMID: 19279259

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Subcellular Location Apical cell membrane; Single-pass type I membrane protein. Endosome lumen. Membrane, coated pit. Cell projection, dendrite. Cell projection, axon.
Protein Families LDLR family
Tissue Specificity In the inner ear, strongly expressed in the marginal cells of the stria vascularis (at protein level). In the female reproductive tract, expressed on the luminal side of the uterine epithelium (at protein level). In the adult brain, expressed in ependymal
Database Links

KEGG: mmu:14725

STRING: 10090.ENSMUSP00000079752

UniGene: Mm.23847

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