Product Details

Purity

>85% (SDS-PAGE)

Target Names

Lrp4

Uniprot No.

Q8VI56

Alternative Names

Lrp4; Kiaa0816Low-density lipoprotein receptor-related protein 4; LRP-4; LDLR dan

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN-induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. Plays an essential role in the process of digit differentiation.

Gene References into Functions

the serum sclerostin levels were strongly increased and the level of sclerostin in the tibia was decreased in Lrp4R1170Q/R1170Q mice, confirming the role of LRP4 as an anchor for sclerostin in bone. PMID: 28477420
The astrocytic Lrp4 plays an important role in ischemic brain injury response. Lrp4 deficiency in astrocytes seems to be protective in response to ischemic brain injury, likely because of the increased ATP release and adenosine-A2AR signaling. PMID: 29212737
LRP4 is expressed by embryonic cortical and hippocampal neurons, and downregulation of LRP4 in these neurons causes a reduction in density of synapses and number of primary dendrites. Collectively, the results demonstrate an essential and novel role of neuronal LRP4 in dendritic development and synaptogenesis in the central nervous system. PMID: 29061639
data suggest a model whereby Lrp4 modulates Wnt/beta-catenin signaling via interaction with Wnt ligands and antagonists in a context-dependent manner. PMID: 28694256
a critical role for Lrp4, in response to agrin, in modulating astrocytic ATP release and synaptic transmission PMID: 27294513
We show that prepatterning in mice requires Lrp4 but not the MuSK Fz-like domain PMID: 27151977
The loss of low density lipoprotein receptor-related protein 4 compromised foetal swallowing and breathing-like movements and downregulated the expression of aquaporin-9 in the foetal membrane and aquaporin-1 in the placenta, which possibly affected the amniotic fluid clearance. PMID: 26847765
Reveal novel roles for APP in regulating neuromuscular synapse formation through hetero-oligomeric interaction with LRP4 and agrin and thereby provide new insights into the molecular mechanisms that govern NMJ formation and maintenance. PMID: 23986861
Results suggest that mdig may contribute to silica-induced lung fibrosis by altering the balance between Th17 and Treg cells. PMID: 25669985
These studies demonstrate a role for Lrp4 in hippocampal function. PMID: 25407677
Lrp4 is a critical player in bone-mass homeostasis. PMID: 25733894
found by laser capture microdissection that LRP4 expression was induced during chondrocyte differentiation in the growth plate PMID: 25091481
pathogenic IgG4 antibodies to MuSK bind to a structural epitope in the first Ig-like domain of MuSK, prevent binding between MuSK and Lrp4, and inhibit Agrin-stimulated MuSK phosphorylation. PMID: 24297891
Mice immunized with the extracellular domain of LRP4 generated anti-LRP4 antibodies & exhibited myasthenia-gravis-associated symptoms & neuromuscular junction abnormality. LRP4 contributes to neuromuscular junction maintenance in adulthood. PMID: 24200689
Lrp4 is essential for patterning of the mammary placodes. PMID: 23293290
Lrp4 acts bidirectionally and coordinates synapse formation by binding agrin, activating MuSK and stimulating postsynaptic differentiation, and functioning in turn as a muscle-derived retrograde signal that is necessary and sufficient for presynaptic differentiation PMID: 22854782
Lrp4, lacking the Lrp4 intracellular region, is sufficient for presynaptic and postsynaptic differentiation. PMID: 22038977
Lrp4 is a critical regulator of ureteric bud branching and lack of Lrp4 results in congenital kidney malformations PMID: 20454682
Modulator of cellular signaling pathways involving Wnt proteins, bone morphogenetic proteins, fibroblast growth factors and sonic hedgehog homolog. PMID: 16207730
The absence of Lrp4 expression in the blastocyst, embryonic stem cells and embryonic germ cells suggests the Lrp4 is a molecular marker that distinguishes the germ cells from embryo-derived pluripotent stem cells. PMID: 16434236
The gene encoding MEGF7/LRP4, a member of the low-density lipoprotein receptor family, plays an essential role in the process of digit differentiation. PMID: 16517118
Data show that Lrp4 is required during the earliest events in postsynaptic neuromuscular junction (NMJ) formation and suggest that it acts in the early, nerve-independent steps of NMJ assembly. PMID: 17119023
These experiments indicate that Lrp4 is the long-sought and elusive receptor for Agrin and has a critical role in activating MuSK and stimulating neuromuscular synapse formation. PMID: 18848351
These observations indicate that LRP4 is a coreceptor of agrin that is necessary for MuSK signaling and AChR clustering and identify a potential target protein whose mutation and/or autoimmunization may cause muscular dystrophies. PMID: 18957220
Lrp4 modulates and integrates Bmp and canonical Wnt signalling during tooth morphogenesis by binding the secreted Bmp antagonist protein Wise PMID: 19116665

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Involvement in disease

Defects in Lrp4 are the cause of digitation anormale (dan) phenotype, this mutation is the consequence of a retroviral insertion. Dan mice shown growth retardation in 10-day-old mice dan/dan and polysyndactyly (PubMed:16517118). Defects in Lrp4 are the cause of malformed digits (mdig) phenotype. It is a spontaneous, autosomal recessive mutation resulting in polysyndactyly (PubMed:16517118).

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

LDLR family

Database Links

KEGG: mmu:228357

STRING: 10090.ENSMUSP00000028689

UniGene: Mm.275149

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Recombinant Mouse Low-density lipoprotein receptor-related protein 4 (Lrp4), partial

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