Product Details

Purity

>85% (SDS-PAGE)

Target Names

Magel2

Uniprot No.

Q9QZ04

Alternative Names

Magel2MAGE-like protein 2; Protein nS7

Species

Mus musculus (Mouse)

Expression Region

1-490

Target Protein Sequence

MFIGATFCAP RGASASRAYV PTAWKNLPAT SETFPATSRV FPSTSHFQPA SSNAFRGPSA ASESPKSLPF ALQDPYACVE ALPAVPWVPY PDGNASSACK SVPAILMVAA AAPQASATAA EASKSSEPPR RPGKATRKKK HLEPKEDNCG HRLSSRDWRG PRTWGNPSHS DWEIQRAMQL LGDRESLYTP QGLNDWGCPN TSRMPRSLEG PSTSRDQEFC GDSGGSQTWM ASEVPSVSRG SSAAQEDPDR ESQPLSPLDE RANALVQFLL VKDQAKVPVQ LSEMVNVVIR EYKDDSLDII NRANTKLECT FGCQLKEVDT KTHTYIIVNK MAYPQCNLLA SYLERPKFSL LMVVLSLIFM KGYCIRENLL FSFLFQLGLD VQETSGLFRI TKKLITSVFV RHRYLEYRQI PFTEPAEYEL LWGPRAFLET NRVHILRFLA ALYENQPQIW SCQYLDSLAE LEYKDANAAA EESHDSDDDA HDPTSSPHPH

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Probably enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases, possibly through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Acts as a regulator of retrograde transport via its interaction with VPS35. Recruited to retromer-containing endosomes and promotes the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1 together with TRIM27, leading to promote endosomal F-actin assembly. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Significantly promotes the cytoplasmic accumulation of CLOCK.

Gene References into Functions

We detected fundamental deficits in the Magel2-null brain, including global decreases in catecholamine and indolamine pathway biogenic amines, with the catecholamine pathway most affected in the hindbrain and hypothalamus. PMID: 27254754
Magel2 knockout mice displayed altered social phenotype and a lack of preference for social novelty. PMID: 28296079
these findings suggest that a loss of Magel2 leads to the disruption of hypothalamic feeding circuits, an effect that appears to be independent of the neurodevelopmental effects of leptin and ghrelin and likely involves a direct neurotrophic effect of Magel2. PMID: 27288456
Normal leptin responses were found in Magel2-null mice up to 4 weeks of age, but the proportion of leptin-responsive POMC neurons was reduced in 6-week-old Magel2-null mice. PMID: 25926624
Magel2 inactivation induces a deficit in social recognition and social interaction and a reduced learning ability in adult male mice. PMID: 25599930
This neural defect, together with increased fat mass, blunted circadian rhythm, and growth hormone response pathway defects that are also linked to loss of MAGEL2, could contribute to the hyperphagia and obesity that are hallmarks of this disorder. PMID: 23341784
This study demonstrated that Magel2-null mice have abnormalities of hypothalamic endocrine axes that recapitulate phenotypes in Prader-Willi syndrome. PMID: 21248145
Magel2-deficient mouse with 50% neonatal mortality had an altered onset of suckling activity and subsequent impaired feeding. PMID: 20876615
Magel2 gene is imprinted, with preferential expression from the paternal allele in mouse and human. PMID: 10915770
role of the circadian rhythm output gene Magel2 in brain structure and behavior PMID: 19199291
necdin and MAGE-G1 target both E2F1 and p75 to regulate cell viability during brain development. PMID: 14593116
Inactivation of the mouse Magel2 gene results in growth abnormalities similar to Prader-Willi syndrome PMID: 17728320
The robust rhythmicity of Magel2 expression in the SCN and the altered behavioral rhythmicity of null mice reveal Magel2 to be a clock-controlled circadian output gene whose disruption results in some of the phenotypes characteristic of PWS. PMID: 17893678

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Subcellular Location

Early endosome. Cytoplasm. Nucleus.

Tissue Specificity

Expressed predominantly in late development stages and adult brain.

Database Links

KEGG: mmu:27385

STRING: 10090.ENSMUSP00000079265

UniGene: Mm.45207

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Recombinant Mouse MAGE-like protein 2 (Magel2)

Product Details

Customer Reviews and Q&A

Target Background

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