Recombinant Mouse Major facilitator superfamily domain-containing protein 2A (Mfsd2a), partial

Code CSB-YP866727MO
MSDS
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Source Yeast
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Code CSB-EP866727MO
MSDS
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Source E.coli
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Code CSB-EP866727MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866727MO
MSDS
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Source Baculovirus
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Code CSB-MP866727MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mfsd2a
Uniprot No.
Alternative Names
Mfsd2a; Mfsd2; Nls1; Sodium-dependent lysophosphatidylcholine symporter 1; NLS1; Sodium-dependent LPC symporter 1; Major facilitator superfamily domain-containing protein 2A
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Sodium-dependent lysophosphatidylcholine (LPC) symporter, which plays an essential role for blood-brain barrier formation and function. Specifically expressed in endothelium of the blood-brain barrier of micro-vessels and transports LPC into the brain. Transport of LPC is essential because it constitutes the major mechanism by which docosahexaenoic acid (DHA), an omega-3 fatty acid that is essential for normal brain growth and cognitive function, enters the brain. Transports LPC carrying long-chain fatty acids such LPC oleate and LPC palmitate with a minimum acyl chain length of 14 carbons. Does not transport docosahexaenoic acid in unesterified fatty acid. Specifically required for blood-brain barrier formation and function, probably by mediating lipid transport. Not required for central nervous system vascular morphogenesis. Acts as a transporter for tunicamycin, an inhibitor of asparagine-linked glycosylation.
Gene References into Functions
  1. This study showed that after injury recovery, the adult liver re-establishes the metabolic zonation by reprogramming the Mfsd2a(+)-derived hepatocytes into pericentral hepatocytes. PMID: 27857132
  2. Mfsd2a may protect against BBB injury by inhibiting vesicular transcytosis following intracerebral hemorrhage. PMID: 28724654
  3. Transplantation of endothelial progenitor cells that overexpress MFSD2A reduce colitis in mice. PMID: 28827082
  4. Maternal DHA supplementation had a strong impact on offspring hepatic gene expression, especially of the fatty acid transporter, Mfsd2a, suggesting a dynamic interplay between DHA synthesis and DHA uptake in the control of systemic levels in the offspring PMID: 27864326
  5. Mfsd2a lipid transport function is required for suppression of transcytosis and blood brain barrier integrity. PMID: 28416077
  6. These findings identify LPC transport via Mfsd2a as an important pathway for DHA uptake in eye and for development of photoreceptor membrane discs. PMID: 27008858
  7. findings identify Mfsd2a as a key regulator of blood brain barrier function that may act by suppressing transcytosis in CNS endothelial cells. PMID: 24828040
  8. identification of a member of the major facilitator superfamily--Mfsd2a (previously an orphan transporter)--as the major transporter for docosahexaenoic acid uptake into brain PMID: 24828044
  9. Data suggest that the ligand(s) that are transported by MFSD2A play important roles in these physiological processes and await future identification. PMID: 23209793
  10. Results identify Mfsd2a (major facilitator superfamily domain-containing protein 2a) and an additional closely related protein Mfsd2b, and suggest that Mfsd2a plays a role in adaptive thermogenesis. PMID: 18694395

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Major facilitator superfamily
Tissue Specificity
Widely expressed. Exhibits an oscillatory pattern of expression in brown adipose tissue and liver consistent with a circadian rhythm. Enriched in brain micro-vessels, where it is specifically present in endothelium constituting the blood-brain barrier (at
Database Links
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