Recombinant Mouse Microtubule-associated protein 1B (Map1b), partial

Code CSB-YP013401MO
MSDS
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Source Yeast
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Code CSB-EP013401MO
MSDS
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Source E.coli
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Code CSB-EP013401MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013401MO
MSDS
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Source Baculovirus
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Code CSB-MP013401MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Map1b
Uniprot No.
Alternative Names
Map1b; Mtap1b; Mtap5Microtubule-associated protein 1B; MAP-1B; MAP1(X); MAP1.2) [Cleaved into: MAP1B heavy chain; MAP1 light chain LC1]
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing. Facilitates tyrosination of alpha-tubulin in neuronal microtubules. Required for synaptic maturation.
Gene References into Functions
  1. Data show that loss of MAP1B function affects general cognitive ability through a profound, brain-wide WM deficit with likely disordered or compromised axons. PMID: 30150678
  2. MAP1B knockout neurons display a decrease in the density of presynaptic and postsynaptic terminals, which involves a reduction in the density of synaptic contacts, and an increased proportion of orphan presynaptic terminals. MAP1B knockout neurons present altered synaptic vesicle fusion events, and a decrease in the density of both synaptic vesicles and dense core vesicles at presynaptic terminals. PMID: 27425640
  3. MAP1B restricts the access of AMPARs to dendritic spines and the postsynaptic membrane, contributing to downregulating synaptic transmission PMID: 28904092
  4. MAP1B confers increased stability to tyrosinated and acetylated, but not detyrosinated microtubules. PMID: 26773468
  5. Both FMRP deficiency in Fmr1(I304N) mice and Fmr1 knockdown impeded the axonal delivery of miR-181d, Map1b, and Calm1 and reduced the protein levels of MAP1B and calmodulin in axons. PMID: 26711345
  6. MAP1B is a specific marker protein of the podocyte microtubular cytoskeleton. PMID: 26448484
  7. The morphology, phenotype, regional distribution, proliferative dynamics, and stage-specific marker expression of Map5 cells in the rabbit and mouse central nervous system, were characterized. PMID: 23667595
  8. The MAP1B-Tiam1-Rac1 relay is essential for spine structural plasticity and removal of AMPA receptors from synapses during long-term depression. PMID: 23881099
  9. MAP1B interacts directly with EB1 and EB3 (EBs), two core 'microtubule plus-end tracking proteins' ( TIPs), and sequesters them in the cytosol of developing neuronal cells. PMID: 23572079
  10. MKK7 is specifically phosphorylated in the neurite shaft which triggers Map1b phosphorylation to regulate microtubule bundling leading to neurite elongation. PMID: 23226105
  11. Mutation of the Map1b binding site of Na(v)1.6 prevented generation of sodium current in transfected cells. The data indicate that Map1b facilitates trafficking of Na(v)1.6 to the neuronal cell surface PMID: 22474336
  12. Microtubule associated protein 1B, a microtubule stabilizing protein, and clathrin heavy chain, the major component of the clathrin triskelion, were identified as interaction partners for dystonin-a PMID: 21936565
  13. important role for MAP1B in the formation and maturation of dendritic spines PMID: 21984824
  14. HuD simultaneously binds both RNA and MAP1B-light chain 1 (MAP1B-LC1) in vitro and that it tightly associates with microtubules in cells in an LC1-dependent manner PMID: 21288476
  15. MAP1S and MAP1B both are involved in regulating trafficking of NR3A-containing NMDAR. PMID: 20304030
  16. Participation of structural microtubule-associated proteins (MAPs) in the development of neuronal polarity. PMID: 11891784
  17. developmentally programmed FMRP expression represses the translation of microtubule associated protein 1B (MAP1B) and is required for the accelerated decline of MAP1B during active synaptogenesis in neonatal brain development PMID: 15475576
  18. Through MAP1B function, Reelin could modify the neuronal cytoskeleton of migration neurons PMID: 15590913
  19. GSK-3beta phosphorylation of MAP1B at Ser1260 and Thr1265 is spatially restricted to growing axons PMID: 15731007
  20. The interactions of MAP1B with LIS1 in hippocampal neurons, and the association of LIS1 with dynein are reported. PMID: 15762842
  21. Mice homozygous for a deletion of the MAP1B gene demonstrate impaired locomotor activity most likely correlated to a lack of physical endurance. Electroretinography indicated a reduction of the a-wave amplitude in response to a flash of white light. PMID: 16102853
  22. MAP1B is associated with the regulation of retrograde axonal transport of mitochondria PMID: 16536727
  23. The 3'UTR of MAP1B mRNA interacts with QKI, and oligodendroglia-specific QKI-deficiency in the quakingviable mutant mice resulted in reduced MAP1B mRNA expression. PMID: 16855020
  24. We conclude, that the MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. PMID: 17292804
  25. Mtap1b-Light chain 1 has the potential to repress cell proliferation by modulating the nucleolar levels of Pes1 PMID: 17308336
  26. GAPDH may be essential in the local energy provision of cytoskeletal structures and MAP1B may help to keep this key enzyme close to the cytoskeleton. PMID: 17521179
  27. These results reveal an S-nitrosylation-dependent signal-transduction pathway that is involved in regulation of the axonal cytoskeleton and identify MAP1B as a major component of this pathway. PMID: 17704770
  28. MAP1B is required for microtubule backfolding, thereby unravelling an important molecular mechanism implicated in coupling the movements of actin and microtubules during process retraction of neural cells. PMID: 17764972
  29. MAP1B regulates tyrosination of alpha-tubulin in neuronal microtubules. This regulation may be important for general processes involved in nervous system development such as axonal guidance and neuronal migration. PMID: 18075266
  30. Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons. PMID: 19549690

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Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm. Cell junction, synapse. Cell projection, dendritic spine.; [MAP1 light chain LC1]: Cytoplasm.
Protein Families
MAP1 family
Database Links
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