Recombinant Mouse Myeloid leukemia factor 1 (Mlf1)

Code CSB-YP014621MO
MSDS
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Source Yeast
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Code CSB-EP014621MO
MSDS
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Source E.coli
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Code CSB-EP014621MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP014621MO
MSDS
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Source Baculovirus
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Code CSB-MP014621MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mlf1
Uniprot No.
Alternative Names
Mlf1; Hls7Myeloid leukemia factor 1; Hematopoietic lineage switch 7; Myelodysplasia-myeloid leukemia factor 1
Species
Mus musculus (Mouse)
Expression Region
1-267
Target Protein Sequence
MFRMLSSSFE DDPFFADSFL AHRESMRNMM RSFSEPLGRD LLSISDGRGR THNRRERDDG EDSLTHADVN PFQTMDRMMA NMRSGIQELQ RNFGQLSMDP NGHSFCSSSV MTYSKVGDEP PKVFQASTQT RRAPGGVKET RKAMRDSDSG LERMAVGHHI HDRGHVIRKS KNNKTGDEEV NQEFINMNES DAHAFDDEWQ NEVLKYKSIG RSGNTGMRSV GHEHPGSREL KRREKIHRNS AIESGRRSNV FVDKLNVKGS PVKITKK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
Gene References into Functions
  1. The data indicate that MLF1 serves as a proapoptotic antagonist that interacts with the HAX1/HtrA2-OMI/PARL (HOP) mitochondrial complex to modulate cell survival. PMID: 28137643
  2. Ectopically expressed MLF1 could be responsible for the pathological apoptosis in early myelodysplastic syndrome (MDS) patients. PMID: 26563351
  3. Mlf1 translocates between nucleus and cytoplasm, associates with a novel hnRNP, and influences gene expression PMID: 17008314
  4. shuttling of MLF1 is critical for the regulation of cell proliferation and a disturbance in the shuttling balance increases the cell's susceptibility to oncogenic transformation PMID: 17967869
Subcellular Location
Cytoplasm. Nucleus. Cell projection, cilium. Cytoplasm, cytoskeleton, cilium basal body.
Protein Families
MLF family
Tissue Specificity
Highly expressed in skeletal muscle, heart, testis. Also found in lung, but not in spleen, thymus, bone marrow, liver and kidney.
Database Links

KEGG: mmu:17349

UniGene: Mm.10414

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