Recombinant Mouse Myocyte-specific enhancer factor 2C(Mef2c)

Code CSB-YP804958MO
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Source Yeast
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Code CSB-EP804958MO
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Source E.coli
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Code CSB-EP804958MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP804958MO
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Source Baculovirus
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Code CSB-MP804958MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Mef2c
Uniprot No. Q8CFN5
Alternative Names Mef2c; Myocyte-specific enhancer factor 2C; Myocyte enhancer factor 2C
Species Mus musculus (Mouse)
Expression Region 1-474
Target Protein Sequence MGRKKIQITR IMDERNRQVT FTKRKFGLMK KAYELSVLCD CEIALIIFNS TNKLFQYAST DMDKVLLKYT EYNEPHESRT NSDIVETLRK KGLNGCDSPD PDADDSVGHS PESEDKYRKI NEDIDLMISR QRLCAVPPPS FEMPVTIPVS SHNSLVYSNP VSTLGNPNLL PLAHPSLQRN SMSPGVTHRP PSAGNTGGLM GGDLTSGAGT SAGNGYGNPR NSPGLLVSPG NLNKNIQAKS PPPMNLGMNN RKPDLRVLIP PGSKNTMPSV SEDVDLLLNQ RINNSQSAQS LATPVVSVAT PTLPGQGMGG YPSAISTTYG TEYSLSSADL SSLSGFNTAS ALHLGSVTGW QQQHLHNMPP SALSQLGACT STHLSQSSNL SLPSTQSLSI KSEPVSPPRD RTTTPSRYPQ HTTRHEAGRS PVDSLSSCSS SYDGSDREDH RNEFHSPIGL TRPSPDERES PSVKRMRLSE GWAT
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Enhances transcriptional activation mediated by SOX18. May also be involved in neurogenesis and in the development of cortical architecture. Isoforms that lack the repressor domain are more active than isoform 1. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells.
Gene References into Functions
  1. This study demonstrated that using multidimensional integrative approaches, we identify MEF2C TF as promising therapeutic target for schizophrenia and other psychiatric disease associated with impaired cognition. PMID: 28115742
  2. A large number of Mef2c targets overlapped with genes down-regulated by Wnt16 and Mef2c itself was transcriptionally repressed by Wnt16 suggesting that Mef2c plays a role in Wnt16-mediated transcriptional regulation. PMID: 29981832
  3. results suggest that MEF2C haploinsufficiency leads to abnormal brain development, E/I imbalance, and neurobehavioral dysfunction, which may be mitigated by pharmacological intervention PMID: 29133852
  4. MiR-204-5p inhibits myoblast differentiation by targeting MEF2C and ERRgamma. PMID: 29505923
  5. MEF2C protects B lymphopoiesis during stress by ensuring proper expression of genes that encode DNA repair and B-cell factors. PMID: 27507714
  6. Enrichment of induced cardiomyocytes derived from mouse fibroblasts can be achieved by reprogramming with cardiac transcription factors, Gata4, MEF2c, Tbx5, and Hand2. PMID: 29257325
  7. MEF2C is a novel target of miR-214-3p in myocardial hypertrophy, and enhancement of miR-214-3p expression may be protective against myocardial hypertrophy. PMID: 27796324
  8. Results show that MEF2C interacts with the N-terminal pre-LIM region of nTRIP6 in proliferating myoblasts. PMID: 27292777
  9. Immune challenge in mice lacking Mef2C in microglia results in an exaggerated microglial response and has an adverse effect on mice behaviour. PMID: 28959042
  10. the expression and the phosphorylation of MEF2Calpha1 are critically required to sustain the adult myogenesis. PMID: 27612437
  11. MEF2C is necessary for Mmp13 gene expression at the transcriptional level and participates in PTH-stimulated Mmp13 gene expression by increased binding to c-FOS at the AP-1 site in the Mmp13 promoter. PMID: 28973134
  12. lf5 ChIP-seq revealed that Klf5 binding overlaps that of MyoD and Mef2, and Klf5 physically associates with both MyoD and Mef2. In addition, MyoD recruitment was greatly reduced in the absence of Klf5. These results indicate that Klf5 is an essential regulator of skeletal muscle differentiation, acting in concert with myogenic transcription factors such as MyoD and Mef2. PMID: 27743478
  13. The authors show here that conditional embryonic deletion of Mef2c in cortical and hippocampal excitatory neurons (Emx1-lineage) produces a dramatic reduction in cortical network activity in vivo, due in part to a dramatic increase in inhibitory and a decrease in excitatory synaptic transmission. Perturbing MEF2C function in neocortex can produce autistic- and intellectual disability-like behaviors in mice. PMID: 27779093
  14. Here, the authors show that loss of Fxn in the nervous system in mice also activates an iron/sphingolipid/PDK1/Mef2 pathway, indicating that the mechanism is evolutionarily conserved. PMID: 27901468
  15. HDAC5 emerges as a cellular conductor of MEF2C and M6a activity and is regulated by miR-124 and miR-9 to control neurite development. PMID: 28332716
  16. In cardiomyocytes exposed to biomechanical stimulation, FAK accumulates in the nucleus, binds to and upregulates the transcriptional activity of MEF2c through an interaction with the FAK focal adhesion targeting (FAT) domain. PMID: 27427476
  17. two MEF2 sites in the enhancer function cooperatively due to bridging of the MEF2C-bound sites by the SAP domain-containing co-activator protein myocardin PMID: 28351867
  18. Our results elucidate the specific role of the transcription factors CREB, SRF, and MEF2 in the depression and potentiation components of ODP in vivo, therefore better informing future attempts to find therapeutic targets for diseases where activity-dependent plasticity is disrupted. PMID: 28607167
  19. that Foxp2-Mef2C signaling is critical to corticostriatal circuit formation PMID: 27595386
  20. Postnatal, postsynaptic deletion of Mef2c in a sparse population of L2/3 neurons suppressed development of excitatory synaptic connections from all local input pathways tested. In the same cell population, Mef2c deletion promoted the strength of excitatory inputs originating from contralateral neocortex. Both the synapse promoting and synapse suppressing effects of Mef2c deletion required normal whisking experience. PMID: 27989458
  21. Critical role for MEF2C in the regulation of spine numbers with a dissociation of learning and memory, synaptic plasticity, and measures of autism-related behaviors in postnatal mouse brain. PMID: 26642739
  22. Endothelial Mef2c regulates the endothelial actin cytoskeleton and inhibits smooth muscle cell migration into the intima. PMID: 28473437
  23. Analysis of SOST expression using large minigenes reveals the MEF2C binding site in the evolutionarily conserved region (ECR5) enhancer mediates forskolin, but not 1,25-dihydroxyvitamin D3 or TGFbeta1 responsiveness. PMID: 26361013
  24. Mef2c is highly expressed in the retina where it modulates photoreceptor-specific gene expression PMID: 28017720
  25. results identify a novel cooperation between MEF2 factors and NR2F2 in the expression of the Akr1c14 gene PMID: 26748576
  26. Data show that three transcriptional factors Gata4, Mef2c, and Tbx5 (abbreviated as GMT) significantly improved murine embryonic stem cells (ESCs) differentiated into cardiomyocytes. PMID: 26449528
  27. MEF2C has a role in regulating outflow tract alignment and transcriptional control of Tdgf1 PMID: 26811383
  28. These findings uncover a novel role for Mef2c/d in coordinating the transcriptional network that promotes early B-cell development. PMID: 26660426
  29. our data indicate that robust hypertrophic MEF2 activation in the heart in vivo requires a background of adiponectin signaling and that adiponectin signaling in primary isolated CM directly enhances MEF2 activity through activation of p38 MAPK PMID: 26196305
  30. SmarcA4 is required for synapse development and myocyte enhancer factor 2-mediated synapse remodeling PMID: 26459759
  31. Our results identify novel target genes for MEF2 and define MEF2 as an important regulator of Leydig cell function and male reproduction. PMID: 26019261
  32. this is a novel mechanism of H2S-mediated activation of MEF2C and induction of miR-133a and inhibition of hypertrophy in HHcy cardiomyocytes. PMID: 25763715
  33. The RBM4-MEF2C-miR-1 network constitutes a novel mechanism which programs the gene expression profile toward the development of brown adipocytes. PMID: 25826570
  34. increased sclerostin production achieved by HDAC5 shRNA is abrogated by simultaneous knockdown of MEF2C, indicating that MEF2C is a major target of HDAC5 in osteocytes PMID: 25271055
  35. Endothelin signaling activates Mef2c expression in the neural crest through a MEF2C-dependent positive-feedback transcriptional pathway. PMID: 26160899
  36. In skeletal muscle, transcription of Lmod3 was controlled by the transcription factors SRF and MEF2. PMID: 25774500
  37. Fox-1, expressed specifically in the neural cell stage, promoted Mef2c exon beta inclusion via the GCAUG. PMID: 20141540
  38. Myocyte enhancer factor 2 C overexpression by adenovirus significantly repressed TNF-alpha induction of pro-inflammatory molecules, activation of NF-kappaB, and leukocyte adhesion to endothelial cells PMID: 25474999
  39. Dlx5 and Mef2 directly bound to the runx2 enhancer, and the binding sites were required for the osteoblast-specific expression PMID: 24692107
  40. TGF-beta1-induced Mmp20 expression in ALCs was regulated through MEF2-binding site on Mmp20 promoter and thus mediated by MEF2C signaling pathway. PMID: 24495128
  41. Data indicate that low-dose parathyroid hormone (PTH) decreased the expression of the myocyte enhancer factor 2C (Mef2c) transcription factor, resulting in decreased sclerostin (Sost) expression in osteoblasts/osteocytes. PMID: 25056116
  42. SRC-2 is critical to transcriptional control modulated by MEF2, GATA-4, and Tbx5, thereby enhancing gene expression associated with cardiac growth. PMID: 24811170
  43. MEF2C is a key effector of the myogenesis program promoted by AUF1. PMID: 24891619
  44. The mRNA expression of Mef2c varies during cardiogenesis in mice, which indicates that Mef2c plays an important role in the process of cardiac development. PMID: 24750843
  45. Chromatin immunoprecipitation analysis confirmed Mef2c binding to the promoters of these genes indicating a direct link between the presence (or absence) of Mef2c and altered transcriptional control in mature B-cells. PMID: 23087187
  46. testosterone induced cardiomyogenesis, at least in part, by recruiting the AR receptor to the regulatory regions of the MEF2C and HCN4 genes. PMID: 23598283
  47. Overexpression of transcription factors MYOCD and SRF alone or in conjunction with Mesp1 and SMARCD3 enhanced the basal but necessary cardio-inducing effect of the previously reported GATA4, TBX5, and MEF2C. PMID: 23704920
  48. Activation of MEF2 reverses the oncogenic properties of cells expressing HDAC4. PMID: 24043307
  49. MEF2C deletion were statistically less likely to have epilepsy. PMID: 23389741
  50. Apelin-APJ signaling plays a novel role as a potent regulator of endothelial MEF2 function in the developing cardiovascular system. PMID: 23603510

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Subcellular Location Nucleus. Cytoplasm, sarcoplasm.
Protein Families MEF2 family
Tissue Specificity Widely expressed though mainly restricted to skeletal and cardiac muscle, brain, neurons and lymphocytes. Beta domain-lacking isoforms are the most predominantly expressed in all tissues including skeletal and cardiac muscle and brain. Only brain expresse
Database Links

KEGG: mmu:17260

STRING: 10090.ENSMUSP00000132547

UniGene: Mm.24001

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