Recombinant Mouse N-acetyltransferase ESCO2 (Esco2)

Code CSB-YP815879MO
MSDS
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Source Yeast
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Code CSB-EP815879MO
MSDS
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Source E.coli
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Code CSB-EP815879MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP815879MO
MSDS
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Source Baculovirus
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Code CSB-MP815879MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Esco2
Uniprot No.
Alternative Names
Esco2N-acetyltransferase ESCO2; EC 2.3.1.-; Establishment of cohesion 1 homolog 2; ECO1 homolog 2
Species
Mus musculus (Mouse)
Expression Region
1-592
Target Protein Sequence
MMATCTPRKR KRYTLNADND DSLLTDISSS KLRCAENLFP SPNKKHNYQS SVQKEDKSCS HQLHFPSSPL KTTENSRFSF ANHSSLFKPA MSTVSFYSKE KYYLNPLERK LIRECRSICL ATESGDKPIP SVTENIQRKP VCTKKNKKKQ KSLTAKYQPN YKHIKSKSRN LKNSKPNQVT YKPVVDQENS CFPAKNYPNS PPRVLSQKIK PQVTLQGGAA FFVRKRNSLK KLPLEDKPLL LQKNLPEVPE GAPEAKQIPK SLLVDEKSSV KVQNARSKNE EKLRKNPSGA VVSSKECNLD KHDFPSENSL DENKTISPES VYPIFNVSSV NTKRPEEQSS VGSTACTNFL KQTNVPKNIN SRDTNKGGKD QLVIDAGQKH FGTTVCKSCG MIYTASNPED EIQHLQHHHR FLEGIKFVGW KRERVVAEFW DGKIVLVLPR DPSYAIKKVE DVQELVDLEL GFQQTVPVCP DKTKTFLFID EKRVVGCLIA EPIKQAFRVL SEPSASKECS RAWRCSDVPE PAICGISRIW VFRLKRRKRI ARRLVDTVRN CFMFGCFLST NEIAFSDPTP DGKLFATKYC NTPNFLVYNF HN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acetyltransferase required for the establishment of sister chromatid cohesion. Couples the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during the S phase. Acetylates the cohesin component SMC3.
Gene References into Functions
  1. ESCO2 knockdown inhibits cell proliferation and induces apoptosis in gastric cancer cells. PMID: 29330052
  2. the involvement of Esco2 in spindle assembly checkpoint and kinetochore functions is mediated by its binding to histone H4 and acetylation of H4K16 both in vivo and in vitro PMID: 28934466
  3. To Roberts syndrome(RBS), mouse Esco2 turns out to be a cell viability factor, the absence of which results in severe chromosome segregation defects and apoptosis. PMID: 22614755
  4. ESCO2 may play a vital role in meiosis in both males and females. PMID: 22699483
  5. Esco2 is needed for cohesin acetylation in PCH and that this modification is required for the proper distribution of cohesin on mitotic chromosomes and for centromeric cohesion PMID: 22101327
  6. Esco2 promotes neuronal differentiation by repressing Notch signaling. PMID: 21777673

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Subcellular Location
Nucleus. Chromosome.
Protein Families
Acetyltransferase family, ECO subfamily
Database Links
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