Recombinant Mouse N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (Napepld)

Code CSB-YP805266MO
MSDS
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Source Yeast
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Code CSB-EP805266MO
MSDS
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Source E.coli
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Code CSB-EP805266MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP805266MO
MSDS
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Source Baculovirus
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Code CSB-MP805266MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Napepld
Uniprot No.
Alternative Names
Napepld; Mbldc1; N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D; N-acyl phosphatidylethanolamine phospholipase D; NAPE-PLD; NAPE-hydrolyzing phospholipase D; EC 3.1.4.54
Species
Mus musculus (Mouse)
Expression Region
1-396
Target Protein Sequence
MDEYEDSQSP APSYQYPKET LRKRQNSVQN SGGSVSSRFS RKSFKLDYRL EEDVTKSKKG KDGRFVNPWP TWKNISIPNV LRWLIMEKNH SGVPGSKEEL DKELPVLKPY FVSDPEDAGV REAGLRVTWL GHATLMVEMD ELIFLTDPMF SSRASPSQYM GPKRFRRPPC TISELPTIDA VLISHNHYDH LDYGSVLALN ERFGSELRWF VPLGLLDWMQ KCGCENVIEL DWWEENCVPG HDKVTFVFTP SQHWCKRTLL DDNKVLWGSW SVLGPWSRFF FAGDTGYCPA FEEIGKRFGP FDLAAIPIGA YEPRWFMKYQ HADPEDAVRI HIDLQTKRSV AIHWGTFALA NEHYLEPPVK LNEALERYGL SCEDFFILKH GESRYLNTDD RAFEET
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
D-type phospholipase that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce bioactive N-acylethanolamines/fatty acid ethanolamides (NAEs/FAEs) and phosphatidic acid. Cleaves the terminal phosphodiester bond of diacyl- and alkenylacyl-NAPEs, primarily playing a role in the generation of long-chain saturated and monounsaturated NAEs in the brain. May control NAPE homeostasis in dopaminergic neuron membranes and regulate neuron survival, partly through RAC1 activation. As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose. Has limited D-type phospholipase activity toward N-acyl lyso-NAPEs.
Gene References into Functions
  1. Data indicate that N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD) knockout mice showed a broad range of lipids that were differentially affected by lipid species and brain region. PMID: 26956082
  2. Adipose tissue NAPE-PLD controls fat mass development by altering the browning process and gut microbiota. PMID: 25757720
  3. Authors demonstrated that phospholipase D and diacylglycerol lipase were required for providing arachidonic acid for prostaglandin E(2) biosynthesis. PMID: 24866789
  4. a major physiological role of NAPE-PLD PMID: 24018423
  5. Phospholipase D is dispensable for superoxide production and degranulation in mouse neutrophils. PMID: 23109426
  6. Suppression of NAPE-PLD expression and palmitoylethanolamide biosynthesis in peritoneal macrophage culture might contribute to an inflammatory response. PMID: 21233218
  7. Characterization of mice lacking N-acyl ethanolamine biosynthetic enzymes. PMID: 20393650
  8. results confirm that overexpressed NAPE-PLD is capable of forming NAEs, including anandamide, in living cells PMID: 15760304
  9. Inactivation of N-acyl phosphatidylethanolamine phospholipase D reveals multiple mechanisms for the biosynthesis of endocannabinoids. PMID: 16605240
  10. Results support the existence of an N-acyl phosphatidylethanolamine (NAPE)-phospholipase D-independent route for N-acyl ethanolamine (NAE) biosynthesis and suggest that Abh4 plays a role in this pathway by acting as a (lyso)NAPE-selective lipase. PMID: 16818490
  11. We suggest that NAEs generated by NAPE-PLD in axons may act as anterograde synaptic signaling molecules that regulate the activity of postsynaptic neurons. PMID: 18067139
  12. NAPE-PLD might be a target to control the activity and excitability of a major sub-population of nociceptive primary sensory neurons. PMID: 19327387

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Subcellular Location
Golgi apparatus membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Nucleus envelope. Nucleus, nucleoplasm.
Protein Families
NAPE-PLD family
Tissue Specificity
Widely expressed. Highest expression in brain, spinal cord, kidney and testis (at protein level). Expressed in adipose tissue (at protein level).
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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