Recombinant Mouse NEDD8 ultimate buster 1 (Nub1)

Code CSB-YP016141MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP016141MO
MSDS
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Source E.coli
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Code CSB-EP016141MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP016141MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP016141MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nub1
Uniprot No.
Alternative Names
Nub1; Nyren18; NEDD8 ultimate buster 1; Negative regulator of ubiquitin-like proteins 1; Protein BS4
Species
Mus musculus (Mouse)
Expression Region
1-614
Target Protein Sequence
MAQKKYLQAK LTQFLREDRI QLWKPPYTKE NKEVGLAVKD LAKKYSERLE CCENEVENII EEIRRKAIER GTGNEHYRTT GIATIEVFLP PRLRKHDKKS LLETRLHVTG RDLRCQIAET FGFQENYIKI VINKKQLQLG KSLEEQGVTH NVKAMVLELK QSEEDVRKNL QLEEEEQNEA ELKERRIQRT KRGLEILAER AEMVVDPETM PYLDIANQTG RSLRIPPAER KALMLAMGYH EKGRAFLKRK EYGIALPCLL DADRYFCECK ELLDTVDNYA VLQLDIVWCY FRLEQLECLD DAEKKLNLAQ KCFKNCYGEN HQRLVHIKGN CGKEKVLFLR LYLLQGIQNY HSGNGEEARE YLNKARQLFK ELYIDPSKVH NLLQLGFTAQ EARLGLRACD GNVDHAATHI SNRREELAQI RKEEKEKRRR RLENVNTLRG MGYSTQAAKQ ALHQARGNLD DALKVLLSNP HMWWLQDADP ENNSRQASPS QESINQLVYM GFDTVVAEAA LRVFGGNVQL AAQTLAHHGG SLPPDLQFSG EDSSPTPSTS PSDSAGTSSA STDEDMETEA VNEILEDIPE HEEDYLDSTL EDEEVIIAEY LSYVESISSA AKNN
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation.
Gene References into Functions
  1. These results suggest that increased levels of NUB1 play a potential role in degradation of detergent-insoluble alpha-synuclein in vivo, although it is insufficient to degrade abnormal alpha-synuclein in Lewy body disease model mice. PMID: 26797281
  2. NUB1L suppresses atypical neddylation and promotes the degradation of misfolded proteins by the proteasome PMID: 26260793
  3. NUB1 has a splicing variant, NUB1L, which regulates the NEDD8 conjugation system PMID: 12816948
Subcellular Location
Nucleus.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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