Recombinant Mouse NFATC2-interacting protein (Nfatc2ip)

Code CSB-YP015747MO
MSDS
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Source Yeast
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Code CSB-EP015747MO
MSDS
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Source E.coli
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Code CSB-EP015747MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015747MO
MSDS
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Source Baculovirus
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Code CSB-MP015747MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nfatc2ip
Uniprot No.
Alternative Names
Nfatc2ip; Nip45NFATC2-interacting protein; 45 kDa NF-AT-interacting protein; 45 kDa NFAT-interacting protein; Nuclear factor of activated T-cells; cytoplasmic 2-interacting protein
Species
Mus musculus (Mouse)
Expression Region
1-412
Target Protein Sequence
MAEPLRGRGP RSRGGRGARR ARGARGRCPR ARQSPARLIP DTVLVDLVSD SDEEVLEVAD PVEVPVARLP APAKPEQDSD SDSEGAAEGP AGAPRTLVRR RRRRLLDPGE APVVPVYSGK VQSSLNLIPD NSSLLKLCPS EPEDEADLTN SGSSPSEDDA LPSGSPWRKK LRKKCEKEEK KMEEFPDQDI SPLPQPSSRN KSRKHTEALQ KLREVNKRLQ DLRSCLSPKQ HQSPALQSTD DEVVLVEGPV LPQSSRLFTL KIRCRADLVR LPVRMSEPLQ NVVDHMANHL GVSPNRILLL FGESELSPTA TPSTLKLGVA DIIDCVVLAS SSEATETSQE LRLRVQGKEK HQMLEISLSP DSPLKVLMSH YEEAMGLSGH KLSFFFDGTK LSGKELPADL GLESGDLIEV WG
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
In T-helper 2 (Th2) cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2. Recruits PRMT1 to the IL4 promoter; this leads to enhancement of histone H4 'Arg-3'-methylation and facilitates subsequent histone acetylation at the IL4 locus, thus promotes robust cytokine expression. Down-regulates formation of poly-SUMO chains by UBE2I/UBC9.
Gene References into Functions
  1. Under standard, non-stressful conditions, FLAG-mNip45 diffusely distributed in the nucleus. PMID: 23159618
  2. results indicate that RANKL signaling down regulates NIP45 expression and that NIP45 is a negative regulator of osteoclast differentiation PMID: 22105856
  3. This finding highlights a possible role of the RENi proteins in the modulation of Ubc9-mediated poly-SUMO formation. PMID: 20077568
  4. NIP45 promotes robust cytokine expression in vivo, which is required for the efficient handling of parasites, acting as a molecular rheostat serving to amplify the type-2 immune response. PMID: 20133688
  5. Data report a role for arginine methylation of NIP45 in regulating cytokine gene transcription in the T helper lymphocyte. PMID: 15327772

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Subcellular Location
Nucleus. Cytoplasm. Note=TRAF1 is associated with a fraction of NFATC2IP in the cytoplasm and prevents its translocation to the nucleus.
Tissue Specificity
Highest level detected in spleen, thymus and testis.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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