Recombinant Mouse Nanos homolog 3 (Nanos3)

Code CSB-YP015438MO
MSDS
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Source Yeast
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Code CSB-EP015438MO
MSDS
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Source E.coli
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Code CSB-EP015438MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015438MO
MSDS
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Source Baculovirus
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Code CSB-MP015438MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nanos3
Uniprot No.
Alternative Names
Nanos3; Nos3; Nanos homolog 3; NOS-3
Species
Mus musculus (Mouse)
Expression Region
1-178
Target Protein Sequence
MGTFNLWTDY LGLARLVGAL HKEEELDVRL DPKPEPKPSS ESQQASKESS AAPERLCSFC KHNGESRAIY QSHVLKDEAG RVLCPILRDY VCPQCGATQE HAHTRRFCPL TSQGYTSVYC YTTRNSAGKK LTRPDKAKTQ DAGHRLGGEA AAGVYAGSKS GRKPPGPSPS ACCPSTTA
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis.
Gene References into Functions
  1. NANOS3 mutation is one possible cause of primary ovarian insufficiency. PMID: 24091668
  2. Study suggests that Nanos3-3'UTR has an essential role in translational control in embryo. PMID: 20174582
  3. Report distinct posttranscriptional regulations in primordial germ cells soon after their specification, for which RNA-binding proteins such as NANOS3 would play critical functions. PMID: 19861488
  4. cloning and functional analyses of nanos2 and nanos3; demonstrated an evolutionarily conserved function of nanos proteins in germ cell development [nanos2] [nanos3] PMID: 12947200
  5. eNOS is the main isoform influencing the initiation of caerulein-induced AP. eNOS-derived NO exerts a protective effect through actions on nonacinar cell types, most likely endothelial cells, to produce greater pancreatic microvascular blood flow. PMID: 14962849
  6. Potential role of Nanos3 in maintaining the undifferentiated spermatogonia population is reported. PMID: 18089289
  7. Results suggest that Nanos3 maintains the germ cell lineage by suppressing both Bax-dependent and Bax-independent apoptotic pathways. PMID: 18436203
  8. detection of the germ cell-specific proteins Nanos2 and Nanos3 as markers of spermatogonia has enabled the clear dissection of complex populations of these cells as Nanos2 was recently shown to be involved in the maintenance of stem cells PMID: 19818747

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Subcellular Location
Nucleus. Cytoplasm. Cytoplasm, Stress granule. Cytoplasm, P-body.
Protein Families
Nanos family
Tissue Specificity
Expressed in undifferentiated spermatogonial cells.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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