Recombinant Mouse Neurexin-3-alpha (Nrxn3), partial

Code CSB-YP761313MO
MSDS
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Source Yeast
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Code CSB-EP761313MO
MSDS
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Source E.coli
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Code CSB-EP761313MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP761313MO
MSDS
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Source Baculovirus
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Code CSB-MP761313MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nrxn3
Uniprot No.
Alternative Names
Nrxn3; Neurexin-3; Neurexin III-alpha; Neurexin-3-alpha
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Neuronal cell surface protein that may be involved in cell recognition and cell adhesion. May mediate intracellular signaling.
Gene References into Functions
  1. In the present study, we report that Nrxn3beta is processed by the metalloproteases ADAM10, ADAM17, and by the intramembrane-cleaving protease gamma-secretase, producing secreted neurexin3beta (sNrxn3beta) and a single intracellular domain (Nrxn3beta-ICD). Thea authors show that sNrxn3beta produced by the cells of the dentate gyrus increases the spine density of newborn neurons. PMID: 27991559
  2. Nrxn3 is responsible for the neuroligins (NLs)-induced assembly of GABAergic synapses in co-culture. Importantly, lentivirus-based expression of Nrxn3 containing exon 20 restores the reduced NLs-induced GABAergic contacts in the SLM2 E/E co-culture. PMID: 28939043
  3. Alternative splicing of presynaptic neurexin-3 controls postsynaptic AMPA receptor trafficking, revealing an unanticipated alternative splicing mechanism for trans-synaptic regulation of synaptic strength and long-term plasticity. PMID: 23827676
  4. N-terminal sequencing of cow neurexin III beta provides evidence for signal peptide PMID: 8163501
  5. N-terminal sequencing of rat neurexin III alpha provides evidence for signal peptide PMID: 9856994
  6. NRXN3 adhesion molecules may play an important role in the synaptic plasticity of neurons involved in the indirect pathways of basal ganglia PMID: 18418251

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Neurexin family
Tissue Specificity
Brain and arteries (at protein level).
Database Links
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