Recombinant Mouse Neutral ceramidase (Asah2), partial

Code CSB-YP881290MO
MSDS
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Source Yeast
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Code CSB-EP881290MO
MSDS
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Source E.coli
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Code CSB-EP881290MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP881290MO
MSDS
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Source Baculovirus
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Code CSB-MP881290MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Asah2
Uniprot No.
Alternative Names
Asah2Neutral ceramidase; N-CDase; NCDase; EC 3.5.1.-; EC 3.5.1.23; Acylsphingosine deacylase 2; N-acylsphingosine amidohydrolase 2) [Cleaved into: Neutral ceramidase soluble form]
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine. Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells. Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells. This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract.
Gene References into Functions
  1. These results suggest that neutral ceramidase is a mediator of necroptosis and might be a novel therapeutic target for protection from ischaemic injury. PMID: 26747710
  2. Knocking down NCDase reduced sphingosine accumulation in mitochondria and preserved COX activity after the brain injury. PMID: 24659784
  3. Neutral ceramidase deficiency increases inflammation in a mouse model of inflammatory bowel disease. PMID: 22940715
  4. Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-CoA. PMID: 21613224
  5. the predicted HNF-4alpha binding site identified in the Asah2 promoter is functional PMID: 20803549
  6. molecular cloning of the mouse neutral ceramidase gene and its promoter analysis PMID: 12435403
  7. Neutral ceramidase secreted by endothelial cells is released in part associated with caveolin-1. PMID: 12921776
  8. the Asah2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract PMID: 16380386
  9. This enzyme is decreased in the small intestine in CFTR transgenic mice with a deletion in CFTR> PMID: 18955860

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Subcellular Location
[Neutral ceramidase]: Cell membrane; Single-pass type II membrane protein. Membrane raft; Single-pass type II membrane protein. Membrane, caveola; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Mitochondrion. Secreted, extracellular exosome.; [Neutral ceramidase soluble form]: Secreted.
Protein Families
Neutral ceramidase family
Tissue Specificity
Widely expressed. Strongly expressed in small intestine and to a lower extent in liver and kidney. Highly expressed in duodenum, jejunum and ileum along the brush border of the small intestine (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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