Product Details

Purity

>85% (SDS-PAGE)

Target Names

Nono

Uniprot No.

Q99K48

Alternative Names

Nono; Non-POU domain-containing octamer-binding protein; NonO protein

Species

Mus musculus (Mouse)

Expression Region

1-473

Target Protein Sequence

MQSNKAFNLE KQNHTPRKHH QHHHQQHHQQ QQQQQQQQPP PPIPANGQQA SSQNEGLTID LKNFRKPGEK TFTQRSRLFV GNLPPDITEE EMRKLFEKYG KAGEVFIHKD KGFGFIRLET RTLAEIAKVE LDNMPLRGKQ LRVRFACHSA SLTVRNLPQY VSNELLEEAF SVFGQVERAV VIVDDRGRPS GKGIVEFSGK PAARKALDRC SEGSFLLTTF PRPVTVEPMD QLDDEEGLPE KLVIKNQQFH KEREQPPRFA QPGSFEYEYA MRWKALIEME KQQQDQVDRN IKEAREKLEM EMEAARHEHQ VMLMRQDLMR RQEELRRMEE LHNQEVQKRK QLELRQEEER RRREEEMRRQ QEEMMRRQQE GFKGTFPDAR EQEIRMGQMA MGGAMGINNR GAMPPAPVPP GTPAPPGPAT MMPDGTLGLT PPTTERFGQA ATMEGIGAIG GTPPAFNRPA PGAEFAPNKR RRY

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Important for the functional organization of GABAergic synapses. Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript. Plays a key role during neuronal differentiation by recruiting TET1 to genomic loci and thereby regulating 5-hydroxymethylcytosine levels. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

Gene References into Functions

Nono overexpression increased the expression of pro-inflammatory cytokines leading to increased atherosclerotic plaque formation. PMID: 29673854
These findings argue that Nono collaborates with Erk signaling to regulate the integrity of bivalent domains and Mouse embryonic stem cell pluripotency. PMID: 27760330
NONO deficiency significantly compromises the response to ionizing radiation-induced DNA damage in the testes, consistent with cell type-specific loss of DSB repair capacity. The results provide evidence that this RNA binding protein significantly contributes to the DNA damage response in vivo. PMID: 28209515
Data indicate involvement of non-POU domain-containing octamer-binding protein (NONO)/paraspeckle protein component 1 (PSPC1)-aldehyde dehydrogenase 1 (NONO/PSPC1-ALDH1A1) in the modulation of Sertoli cells (SCs) response to mono-(2-ethylhexyl) phthalate (MEHP) challenge. PMID: 28117896
This study provides an improved understanding of the molecular basis (structure and dynamics) that governs the binding of the p54(nrb)/NonO RRM1 to one of its target RNAs. PMID: 27064654
mechanisms have therapeutic implications for reducing beta-cell proliferation in insulinomas by inhibiting phospho-HLXB9 or its interaction with Nono and modulating the expression of its direct (Cblb) or indirect (c-Met) targets PMID: 26342078
We analyzed the expression of sperm-specific Akap3 and the potential regulatory factors of its protein synthesis during mouse spermiogenesis. PMID: 24648398
NONO genetic insufficiency led to upregulation of PSPC1, which replaced NONO in a stable complex with SFPQ. PMID: 25100870
Dsta indicate that NONO bound to p16-Ink4A cell cycle checkpoint gene and potentiated its circadian activation in a PER protein-dependent fashion. PMID: 23267082
p54nrb is a target of the peptidylprolyl isomerase Pin1, suggesting that it may be regulated by phosphorylation-dependent conformational changes as many other nuclear proteins upon entry into mitosis PMID: 15701524
identified two proteins, NONO and WDR5, that can associate with the mammalian PER1 protein; data suggest that NONO probably operates antagonistically to PER proteins in mammalian cells, and that it is essential to normal circadian rhythmicity PMID: 15860628
PSPC1, NONO, and SFPQ form complexes with each other in Sertoli cells and may regulate androgen receptor-mediated transcriptional activity PMID: 16641145
Non-POU-domain-containing, octamer-binding protein NonO overexpression downregulates COX-2 promoter activity in pancreatic-beta RINm5F cells. PMID: 18518878

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Subcellular Location

Nucleus. Nucleus, nucleolus. Nucleus speckle. Chromosome.

Tissue Specificity

Expressed in liver and suprachiasmatic nuclei, hippocampus and neocortex (at protein level). Expression is strongest in neurons in CA1 and CA3 pyramidal regions and granule cells of the dentate gyrus. Detected in testis and kidney.

Database Links

KEGG: mmu:53610

STRING: 10090.ENSMUSP00000033673

UniGene: Mm.280069

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Recombinant Mouse Non-POU domain-containing octamer-binding protein (Nono)

Product Details

Customer Reviews and Q&A

Target Background

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