Recombinant Mouse Nucleoside diphosphate kinase homolog 5 (Nme5)

Code CSB-YP859984MO
MSDS
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Source Yeast
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Code CSB-EP859984MO
MSDS
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Source E.coli
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Code CSB-EP859984MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP859984MO
MSDS
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Source Baculovirus
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Code CSB-MP859984MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nme5
Uniprot No.
Alternative Names
Nme5; Nucleoside diphosphate kinase homolog 5; NDK-H 5; NDP kinase homolog 5; nm23-M5
Species
Mus musculus (Mouse)
Expression Region
1-211
Target Protein Sequence
MEVSMPLPQI YVEKTLALIK PDVVDKEEEI QDIILGSGFT IIQRRKLHLS PEHCSNFYVE QYGKMFFPNL TAYMSSGPLV AMILARHKAI SYWKELMGPS NSLVAKETHP DSLRAIYGTD ELRNALHGSN DFAASEREIR FMFPAVIIEP IPIGQAAKDY INLYVAPTLL QGLTELCKEK PPDPYLWLAD WLMKNNPNKP KLCHFPVTEE P
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Does not seem to have NDK kinase activity. Confers protection from cell death by Bax and alters the cellular levels of several antioxidant enzymes including Gpx5. May play a role in spermiogenesis by increasing the ability of late-stage spermatids to eliminate reactive oxygen species.
Gene References into Functions
  1. Situs inversus, hydrocephalus, suppurative rhinosinusitis,and sperm flagellar defects in Nme5 null mice are indicative of defective ciliogenesis PMID: 21746835
  2. nm23-M5 gene plays an important role in late spermiogenesis by elevating the ability of late-stage spermatids to eliminate reactive oxygen species PMID: 12788088
  3. results reveal that expression of Nm23-M5 plays a critical role in spermiogenesis by increasing the cellular levels of GPX-5 to eliminate reactive oxygen species PMID: 19303412
  4. Nme5 is most abundantly expressed in tissues rich in highly ciliated cells, such as olfactory sensory neurons, and is predicted to be important to cilia. PMID: 17971504
Subcellular Location
Cell projection, cilium.
Protein Families
NDK family
Tissue Specificity
Expressed in the trachea, ependymal cells and oviduct (at protein level). Expressed predominantly in germ cells of the testis. Not expressed in testicular somatic cells.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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