Recombinant Mouse POU domain, class 3, transcription factor 3 (Pou3f3)

Code CSB-YP018398MO
MSDS
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Source Yeast
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Code CSB-EP018398MO
MSDS
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Source E.coli
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Code CSB-EP018398MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018398MO
MSDS
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Source Baculovirus
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Code CSB-MP018398MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pou3f3
Uniprot No.
Alternative Names
Pou3f3; Brn-1; Brn1; Otf8; POU domain; class 3; transcription factor 3; Brain-specific homeobox/POU domain protein 1; Brain-1; Brn-1; Octamer-binding protein 8; Oct-8; Octamer-binding transcription factor 8; OTF-8
Species
Mus musculus (Mouse)
Expression Region
1-497
Target Protein Sequence
MATAASNPYL PGNSLLTAGS IVHSDAAGAG GGGGGGGGGG GGAGGGGGGM QPGSAAVTSG AYRGDPSSVK MVQSDFMQGA MAASNGGHML SHAHQWVTAL PHAAAAAAAA AAAAVEASSP WSGSAVGMAG SPQQPPQPPP PPPQGPDVKG GAGREDLHAG TALHHRGPPH LGPPPPPPHQ GHPGGWGAAA AAAAAAAAAA AAAHLPSMAG GQQPPPQSLL YSQPGGFTVN GMLSAPPGPG GGGGGAGGGA QSLVHPGLVR GDTPELAEHH HHHHHHAHPH PPHPHHAQGP PHHGGGGAGP GLNSHDPHSD EDTPTSDDLE QFAKQFKQRR IKLGFTQADV GLALGTLYGN VFSQTTICRF EALQLSFKNM CKLKPLLNKW LEEADSSTGS PTSIDKIAAQ GRKRKKRTSI EVSVKGALES HFLKCPKPSA QEITNLADSL QLEKEVVRVW FCNRRQKEKR MTPPGIQQQT PDDVYSQVGT VSADTPPPHH GLQTSVQ
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3').
Gene References into Functions
  1. The results of the present study confirm the important function of POU3F3 in nephron patterning, especially in development of the the Thick Ascending Limb of the Loop of Henle. Missense Mutation of POU Domain Class 3 Transcription Factor 3 in Pou3f3L423P Mice Causes Reduced Nephron Number and Impaired Development of the Thick Ascending Limb of the Loop of Henle. PMID: 27420727
  2. Line Pou3f3L423P is a viable mouse model harboring a homozygous Pou3f3 mutation. PMID: 27003440
  3. Brn1 predominantly forms homomdimers on MORE cis elements in NS5 mouse neural stem cells PMID: 26265007
  4. Onset of Pou3f3 expression occurs in ventricular zone progenitors, and that Pou3f3 subsequently label neural progeny switching from deep-layer Ctip2+ identity to Satb2+ upper-layer fate as they migrate to proper superficial positions. PMID: 22892427
  5. Brn-1 and Brn-2 share crucial roles in the production and positioning of mouse neocortical neurons PMID: 12130536
  6. These results suggest that Brn1 is essential for both the development and function of the nephron in the kidney. PMID: 12925600
  7. Brn-1 was shown to be capable of fully replacing the related Oct-6 in Schwann cell development. PMID: 15713637

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Subcellular Location
Nucleus.
Protein Families
POU transcription factor family, Class-3 subfamily
Tissue Specificity
Brain.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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