Recombinant Mouse Phospholipase DDHD1 (Ddhd1)

Code CSB-YP768913MO
MSDS
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Source Yeast
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Code CSB-EP768913MO
MSDS
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Source E.coli
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Code CSB-EP768913MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP768913MO
MSDS
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Source Baculovirus
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Code CSB-MP768913MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ddhd1
Uniprot No.
Alternative Names
Ddhd1Phospholipase DDHD1; EC 3.1.1.-; DDHD domain-containing protein 1; Phosphatidic acid-preferring phospholipase A1 homolog; PA-PLA1
Species
Mus musculus (Mouse)
Expression Region
1-547
Target Protein Sequence
MDEVYLYSDA TTSKIARTVT QKLGFSKASS SGTRLHRGYV EEATLEDKPS QTSHIVFVVH GIGQKMDQGR IIKNTAMMRE AARKMEEKHF SNHATHVEFL PVEWRSKLTL DGDTVDSITP DKVRGLRDML NSSAMDIMYY TSPLYRDELV KGLQQELNRL YSLFCSRNPD FEEKGGKVSI VSHSLGCVIT YDIMMGWNPG GLYEQLLQKE EELPDERWMS YEERHLLDEL YITKRRLREI EDRLHGLKAP SISQTPALKF KVENFFCMGS PLAVFLALRG IRPGNSGSQD HILPREICNR LLNIFHPTDP VAYRLEPLIL KHYSNISPVQ IHWYNTSNPL PYEHMKPNFL NPAKEPTSVS DSENIAAIPS PVTSPVLSRR HYGESITNIG KASILGAASI GKGLGGMLFS RFGRSSASQP SEPSKDSLED DKKPSASPST TTVATQTLPH SGSGFLDSAY FRLQESFFYL PQLLFPENVM QSKDDSLVEL EHRIDFELRE GLVESRYWSA VTSHTAYWSS LDVALFLLTF MYKHEHDTEA KPSLGSL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Phospholipase that hydrolyzes phosphatidic acid, including 1,2-dioleoyl-sn-phosphatidic acid. The different isoforms may change the substrate specificity. Required for the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER).
Gene References into Functions
  1. a possible mechanism of PA regulation of the mitochondrial membrane and demonstrate an in vivo function of PA-PLA1 in the organization of mitochondria during spermiogenesis. PMID: 24599962
Subcellular Location
Cytoplasm.
Protein Families
PA-PLA1 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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