Recombinant Mouse Pleckstrin homology domain-containing family A member 2 (Plekha2)

Code CSB-YP861697MO
MSDS
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Source Yeast
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Code CSB-EP861697MO
MSDS
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Source E.coli
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Code CSB-EP861697MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861697MO
MSDS
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Source Baculovirus
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Code CSB-MP861697MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Plekha2
Uniprot No.
Alternative Names
Plekha2; Tapp2; Pleckstrin homology domain-containing family A member 2; PH domain-containing family A member 2; PH domain-containing adaptor PHAD47; Tandem PH domain-containing protein 2; TAPP-2
Species
Mus musculus (Mouse)
Expression Region
1-425
Target Protein Sequence
MPYVDRQNRI CGFLDIEDNE NSGKFLRRYF ILDTQANCLL WYMDNPQNLA VGAGAVGSLQ LTYISKVSIA TPKQKPKTPF CFVINALSQR YFLQANDQKD LKDWVEALNQ ASKITVPKAG TVPLATEVLK NLTAPPTLEK KPQVAYKTEI IGGVVVQTPI SQNGGDGQEG CEPGTHAFLR RSQSYIPTSG CRPSTGPPLI KSGYCVKQGN VRKSWKRRFF ALDDFTICYF KCEQDREPLR TIPLKDVLKT HECLVKSGDL LMRDNLFEII TTSRTFYVQA DSPEDMHSWI EGIGAAVQAL KCHPREPSFS RSISLTRPGS STLTSAPNSI LSRRRPPAEE KRGLCKAPSV ASSWQPWTPV PQAEEKPLSV EHAPEDSLFM PNPGESTATG VLASSRVRHR SEPQHPKEKP FVFNLDDENI RTSDV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane.
Gene References into Functions
  1. this study shows that TAPP1/2 double knock-out mice develop chronic germinal centers with age and show abnormal expression of B-cell activation and develop lupus-like characteristics including anti-DNA antibodies and deposition of immune complexes in kidneys PMID: 27859053
  2. interacts with phosphatidylinositol (3,4)-bisphosphate thus regulating B-cell activation and autoantibody production PMID: 22777911
Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Note=Locates to the plasma membrane after treatments that stimulate the production of PtdIns3,4P2.
Database Links

KEGG: mmu:83436

UniGene: Mm.261122

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